5α-Tetrahydrocorticosterone

Name: 5α-Tetrahydrocorticosterone
Brand: TargetMol
SKU: T211720
Rating: 4.5 (1 reviews)

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Catalog No. T211720Cas No. 600-63-5

Alias 5α-THB

5α-Tetrahydrocorticosterone (5α-HB) is an endogenous steroid that acts as an agonist of the glucocorticoid receptor (GR) and a metabolite of corticosterone. It serves as an effective topical anti-inflammatory agent in vivo. In rat liver cells, it decreases the binding of metabolites to the glucocorticoid receptor-corticosterone and its 5α-reduced metabolites, with a Kd value of 268 nM. 5α-Tetrahydrocorticosterone is applicable in research on inflammatory skin diseases.

5α-Tetrahydrocorticosterone

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Catalog No. T211720Alias 5α-THBCas No. 600-63-5

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 mg	Inquiry	10-14 weeks	10-14 weeks
50 mg	Inquiry	10-14 weeks	10-14 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	5α-Tetrahydrocorticosterone (5α-HB) is an endogenous steroid that acts as an agonist of the glucocorticoid receptor (GR) and a metabolite of corticosterone. It serves as an effective topical anti-inflammatory agent in vivo. In rat liver cells, it decreases the binding of metabolites to the glucocorticoid receptor-corticosterone and its 5α-reduced metabolites, with a Kd value of 268 nM. 5α-Tetrahydrocorticosterone is applicable in research on inflammatory skin diseases.
In vitro	5α-Tetrahydrocorticosterone, at concentrations ranging from 0.001 to 100 μM, reduces the binding of metabolites to glucocorticoid receptor-corticosterone and its 5α-reduced metabolites in rat hepatocytes, with a Kd value of 268 nM. At a concentration of 1 μM for 16 hours, it enhances the expression of TAT and PEPCK mRNA in H4IIE cells. Additionally, 5α-Tetrahydrocorticosterone, when used at 1-10000 nM over 7 days, inhibits angiogenesis in mouse aortic rings in a concentration-dependent manner, with an EC50 value of 2512 nM. At 3 μM, it increases the levels of Mcp1 and Per1, while it suppresses the expression of Pecam1 in aortic rings.
In vivo	5α-Tetrahydrocorticosterone (administered at 5 mg/kg via intraperitoneal injection, single dose) exhibits glucocorticoid-like effects in mice that have undergone bilateral adrenalectomy. Additionally, 5α-Tetrahydrocorticosterone (applied topically in doses of 25-200 μg over 28 days) effectively inhibits ear swelling induced by croton oil in mice.
Synonyms	5α-THB

Chemical Properties

Molecular Weight	350.49
Formula	C₂₁H₃₄O₄
Cas No.	600-63-5
Smiles	C[C@@]12[C@@]3([C@]([C@]4([C@](C)(C[C@@H]3O)[C@@H](C(CO)=O)CC4)[H])(CC[C@]1(C[C@H](O)CC2)[H])[H])[H]

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc