IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 μg | In stock | $ 106.00 | |
20 μg | 5 days | $ 165.00 | |
50 μg | 5 days | $ 290.00 | |
100 μg | 5 days | $ 447.00 | |
200 μg | 5 days | $ 791.00 | |
500 μg | 5 days | $ 1,670.00 |
Biological Information |
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.2-2 ng/mL.
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Description | IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2. |
Species | Human |
Expression System | HEK293 Cells |
Tag | Tag Free |
Accession Number | P29459-1&NP_002178.2 |
Synonyms | IMD29, CLMF, IL12, NFSK, IL-12A, interleukin 12A, NKSF1, IL12 p70, IL-12B, P35, NKSF2, CLMF2, NKSF, IMD28 |
Construction | The Human IL12A (P29459-1)(Met1-Ser219) was expressed, constructed the plasmid 1; The Human IL12B (NP_002178.2)(Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the Human IL12A&IL12B heterodimer was purified. |
Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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Molecular Weight | 57.2 kDa (predicted) |
Endotoxin | < 5 EU/mg of the protein. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-12 Protein, Human, Recombinant IMD29 IL 12 IMD-29 CLMF NKSF 2 NKSF-2 IL12 NFSK P-35 IMD 28 IMD 29 CLMF-2 IL-12 IL-12A interleukin 12A NKSF-1 CLMF 2 NKSF1 NKSF 1 P 35 IL12 p70 IL-12B IMD-28 P35 NKSF2 CLMF2 NKSF IMD28 recombinant recombinant-proteins proteins protein