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Synonyms: P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $97 | 7-10 days | 7-10 days | |
| 10 μg | $159 | In Stock | In Stock | |
| 20 μg | $247 | 7-10 days | 7-10 days | |
| 50 μg | $441 | 7-10 days | 7-10 days | |
| 100 μg | $705 | 7-10 days | 7-10 days | |
| 200 μg | Inquiry | 7-10 days | 7-10 days | |
| 500 μg | Inquiry | 7-10 days | 7-10 days |
| Description | IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | P29459-1&NP_002178.2 |
| Construction | A DNA sequence encoding the human IL12A (P29459-1)(Met1-Ser219) was expressed, constructed the plasmid 1; A DNA sequence encoding the human IL12B (NP_002178.2)(Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the human IL12A&IL12B heterodimer was purified. Predicted N terminal: Arg23&Ile23 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. https://cdn.targetmol.com/group3/M00/12/F4/CgoaEGZG00mEW0p7AAAAAHi18XI668.png,https://cdn.targetmol.com/group3/M00/13/65/CgoaEWZG0yqEO_14AAAAAMUbn0o043.png |
| Endotoxin | < 5 EU/mg of the protein. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF |
| Molecular Weight | 57.2 kDa (predicted); 34 & 41 kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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