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IL-12 Protein, Human, Recombinant

(Synonyms: P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF) Copy Product Info

Synonyms: P35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF

Catalog No. TMPY-04863 Copy Product Info
IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$977-10 days7-10 days
10 μg$159In StockIn Stock
20 μg$2477-10 days7-10 days
50 μg$4417-10 days7-10 days
100 μg$7057-10 days7-10 days
200 μgInquiry7-10 days7-10 days
500 μgInquiry7-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:SDS-PAGE: 97.2%; SEC-HPLC: 95.5%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Description
IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.
Species
Human
Expression System
HEK293 Cells
TagTag Free
Accession NumberP29459-1&NP_002178.2
ConstructionA DNA sequence encoding the human IL12A (P29459-1)(Met1-Ser219) was expressed, constructed the plasmid 1; A DNA sequence encoding the human IL12B (NP_002178.2)(Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the human IL12A&IL12B heterodimer was purified. Predicted N terminal: Arg23&Ile23
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
https://cdn.targetmol.com/group3/M00/12/F4/CgoaEGZG00mEW0p7AAAAAHi18XI668.png,https://cdn.targetmol.com/group3/M00/13/65/CgoaEWZG0yqEO_14AAAAAMUbn0o043.png
Endotoxin< 5 EU/mg of the protein.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsP35, NKSF2, NKSF1, NKSF, NFSK, interleukin 12A, IMD29, IMD28, IL-12B, IL-12A, IL12 p70, IL12, CLMF2, CLMF
Chemical Properties
Molecular Weight57.2 kDa (predicted); 34 & 41 kDa (reducing conditions)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Keywords

Related Tags: IL-12 Protein, Human, Recombinant chemical structure | IL-12 Protein, Human, Recombinant molecular weight