IL-12 Protein, Human, Recombinant

Catalog No. TMPY-04863

IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.

IL-12 Protein, Human, Recombinant

Catalog No. TMPY-04863

IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.

Pack Size	Price	Availability
5 μg	$66	7-10 days
10 μg	$106	In Stock
20 μg	$165	7-10 days
50 μg	$290	7-10 days
100 μg	$447	7-10 days
200 μg	$791	7-10 days
500 μg	$1,670	7-10 days

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Product Information

Biological Activity	Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.2-2 ng/mL.
Description	IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P29459-1&NP_002178.2
Synonyms	P35,NKSF2,NKSF1,NKSF,NFSK,interleukin 12A,IMD29,IMD28,IL-12B,IL-12A,IL12 p70,IL12,CLMF2,CLMF
Construction	A DNA sequence encoding the human IL12A (P29459-1)(Met1-Ser219) was expressed, constructed the plasmid 1; A DNA sequence encoding the human IL12B (NP_002178.2)(Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the human IL12A&IL12B heterodimer was purified. Predicted N terminal: Arg23&Ile23
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Molecular Weight	57.2 kDa (predicted); 34 & 41 kDa (reducing conditions)
Endotoxin	< 5 EU/mg of the protein.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.