Shopping Cart
Remove All
  • TargetMol
    Your shopping cart is currently empty

FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His), Biotinylated

(Synonyms: Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha) Copy Product Info

Synonyms: Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha

Catalog No. TMPY-05148 Copy Product Info
FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 44 kDa and the accession number is P13599&P07151.

Product Introduction

Bioactivity
Bioactivity
Recombinant Rat FCGRT & B2M Heterodimer Protein, Biotinylated (Cat#TMPY-05148) captured on Biotin CAP-Series S Sensor chip, can bind anti-ERBB2(Trastuzumab biosimilar) with an affinity constant of 77.200 nM as determined in an SPR assay (Biacore 8K) (QC tested).
Description
FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 44 kDa and the accession number is P13599&P07151.
Species
Rat
Expression System
HEK293 Cells
TagC-His
Accession NumberP13599&P07151
ConstructionA DNA sequence encoding the extracellular domain (Met1-Ser298) of rat FCGRT (P13599) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat B2M (P07151) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified protein was biotinylated in vitro. Predicted N terminal: Ala 23 & Ile 21
Protein Purity
> 85 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsFc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha
Chemical Properties
Molecular Weight44 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Calculator

  • Reconstitution Calculator
  • Recombinant Protein Dilution Calculator
  • Specific Activity Calculator

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Related Tags: FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His), Biotinylated chemical structure | FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His), Biotinylated molecular weight