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Synonyms: Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $137 | 7-10 days | 7-10 days | |
| 10 µg | $230 | 7-10 days | 7-10 days | |
| 20 µg | $383 | 7-10 days | 7-10 days | |
| 50 µg | $629 | 7-10 days | 7-10 days | |
| 100 µg | $1,050 | 7-10 days | 7-10 days |
| Bioactivity | Recombinant Rat FCGRT & B2M Heterodimer Protein, Biotinylated (Cat#TMPY-05148) captured on Biotin CAP-Series S Sensor chip, can bind anti-ERBB2(Trastuzumab biosimilar) with an affinity constant of 77.200 nM as determined in an SPR assay (Biacore 8K) (QC tested). |
| Description | FCGRT & B2M Heterodimer Protein, Rat, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 44 kDa and the accession number is P13599&P07151. |
| Species | Rat |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P13599&P07151 |
| Construction | A DNA sequence encoding the extracellular domain (Met1-Ser298) of rat FCGRT (P13599) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat B2M (P07151) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified protein was biotinylated in vitro. Predicted N terminal: Ala 23 & Ile 21 |
| Protein Purity | > 85 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha |
| Molecular Weight | 44 kDa (predicted) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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