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FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His & Avi), Biotinylated
(Synonyms: FcRn, Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha) Copy Product InfoSynonyms: FcRn, Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha
Catalog No. TMPY-05649 Copy Product Info
FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 46 kDa and the accession number is Q61559.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $167 | 7-10 days | 7-10 days | |
| 10 μg | $277 | 7-10 days | 7-10 days | |
| 20 μg | $462 | 7-10 days | 7-10 days | |
| 50 μg | $746 | 7-10 days | 7-10 days | |
| 100 μg | $1,230 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Measured by its ability to bind recombinant rhFc(CHO) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 0.4 nM as determined in a BLI assay. |
| Description | FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 46 kDa and the accession number is Q61559. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | His, Avi |
| Accession Number | Q61559 |
| Construction | A DNA sequence encoding the mouse FCGRT (NP_034319.2) (Met 1-Ser 297) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ser 22 & Ile 21 |
| Protein Purity | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | FcRn, Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha |
| Molecular Weight | 46 kDa (predicted) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His & Avi), Biotinylated chemical structure | FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His & Avi), Biotinylated molecular weight
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