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CD73 Protein, Human, Recombinant (His)

Catalog No. TMPY-01851
Synonyms: eN, NT5, E5NT, NT, 5'-nucleotidase, ecto (CD73), NTE, CALJA, eNT, CD73

CD73 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P21589-1.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CD73 Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 416.00
100 μg 5 days $ 625.00
200 μg 5 days $ 938.00
500 μg 5 days $ 2,490.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information 1. Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) bound MEDI9447 was 0.3 nM. 2. Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP). The specific activity is > 15,000 pmoles/min/μg.

Description CD73 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P21589-1.
Species Human
Expression System HEK293 Cells
Tag C-His
Accession Number P21589-1
Synonyms eN, NT5, E5NT, NT, 5'-nucleotidase, ecto (CD73), NTE, CALJA, eNT, CD73
Construction The Human NT5E (NP_002517.1) (Met1-Lys547) was expressed fused with a polyhistidine tag at the C-terminus.
Protein Purity > 85 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Molecular Weight 59.2 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing Tris, NaCl and CaCl2, pH 7.5, with Glycerol as protectants. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.

References and Literature

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Keywords

CD73 Protein, Human, Recombinant (His) CD-73 eN CD 73 NT5 E5NT 5'-nucleotidase, ecto NT 5 NT 5'-nucleotidase, ecto (CD73) NTE CALJA eNT CD73 NT-5 recombinant recombinant-proteins proteins protein

 

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