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CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)

(Synonyms: Nt5, NT, eNT, CD73, AI447961, 5'-nucleotidase, ecto (CD73), 2210401F01Rik) Copy Product Info

Synonyms: Nt5, NT, eNT, CD73, AI447961, 5'-nucleotidase, ecto (CD73), 2210401F01Rik

Catalog No. TMPY-01342 Copy Product Info
CD73 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q0VEE0.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$47-In Stock
10 μg$74-In Stock
20 μg$118-In Stock
50 μg$228-In Stock
100 μg$4017-10 days7-10 days
200 μg$7007-10 days7-10 days
500 μg$1,4607-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:92.9%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >10,000 pmol/min/µg.
Description
CD73 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q0VEE0.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberQ61503
ConstructionTrp29-Lys549
Protein Purity
> 95 % as determined by SDS-PAGE
CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation1. Lyophilized from 20 mM Tris, 500 mM NaCl, pH 7.4. <br> 2. Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). <br> Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsNt5, NT, eNT, CD73, AI447961, 5'-nucleotidase, ecto (CD73), 2210401F01Rik
Research Background
Chemical Properties
Molecular Weight59.4 kDa (predicted); 59.4 kDa (reducing conditions)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His) chemical structure | CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His) molecular weight