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CD73/5'-Nucleotidase Protein, Cynomolgus, Recombinant (His)
(Synonyms: 5'-nucleotidase, ecto (CD73)) Copy Product InfoSynonyms: 5'-nucleotidase, ecto (CD73)
Catalog No. TMPY-03377 Copy Product Info
CD73 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is EHH53214.1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $75 | - | In Stock | |
| 10 μg | $118 | - | In Stock | |
| 20 μg | $196 | - | In Stock | |
| 50 μg | $386 | - | In Stock | |
| 100 μg | $645 | 7-10 days | 7-10 days | |
| 200 μg | $1,080 | 7-10 days | 7-10 days | |
| 500 μg | $2,120 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:88.3%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >20,000 pmol/min/µg. |
| Description | CD73 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is EHH53214.1. |
| Species | Cynomolgus |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | EHH53214.1 |
| Construction | A DNA sequence encoding the cynomolgus NT5E (EHH53214.1) (Met1-Lys547) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Trp 27 |
| Protein Purity | > 90 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 0.12 M NaCl, 4 mM CaCl2, 20% glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | 5'-nucleotidase, ecto (CD73) |
| Research Background |
| Molecular Weight | 59.2 kDa (predicted); 59 kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD73/5'-Nucleotidase Protein, Cynomolgus, Recombinant (His) chemical structure | CD73/5'-Nucleotidase Protein, Cynomolgus, Recombinant (His) molecular weight
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