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CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated

(Synonyms: FCRIIIb, FCRIII, FCR-10, FCGR3, FCG3, Fc γ RIIIb, Fc gamma RIIIb, Fc fragment of IgG receptor IIIb, CD16b, CD16) Copy Product Info

Synonyms: FCRIIIb, FCRIII, FCR-10, FCGR3, FCG3, Fc γ RIIIb, Fc gamma RIIIb, Fc fragment of IgG receptor IIIb, CD16b, CD16

Catalog No. TMPY-06550 Copy Product Info
CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is AAA35881.1.

Product Introduction

Bioactivity
Bioactivity
Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 μM as determined in SPR assay (Biacore T200).
Description
CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is AAA35881.1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberAAA35881.1
ConstructionGly17-Ser200
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsFCRIIIb, FCRIII, FCR-10, FCGR3, FCG3, Fc γ RIIIb, Fc gamma RIIIb, Fc fragment of IgG receptor IIIb, CD16b, CD16
Research Background
Chemical Properties
Molecular Weight23.81 kDa (Predicted); 43-53 kDa (Reducing conditions due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated chemical structure | CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated molecular weight