CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated

Catalog No. TMPY-06550

CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is AAA35881.1.

CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated

Catalog No. TMPY-06550

CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is AAA35881.1.

Pack Size	Price	USA Warehouse	Global Warehouse
5 μg	$215	7-10 days	7-10 days
10 μg	$359	7-10 days	7-10 days
20 μg	$600	7-10 days	7-10 days
50 μg	$939	7-10 days	7-10 days
100 μg	$1,580	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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Product Information

Biological Activity	Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 μM as determined in SPR assay (Biacore T200).
Description	CD16b Protein, Human, Recombinant (NA1, His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is AAA35881.1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	AAA35881.1
Synonyms	FCRIIIb,FCRIII,FCR-10,FCGR3,FCG3,Fc γ RIIIb,Fc gamma RIIIb,Fc fragment of IgG receptor IIIb,CD16b,CD16
Construction	Gly17-Ser200
Protein Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Molecular Weight	23.81 kDa (Predicted); 43-53 kDa (Reducing conditions due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.