Tubulin polymerization-IN-26 (compound 12h) is a chemical compound that inhibits the polymerization of microtubulin by binding to the colchicine binding site, with an IC50 value of 4.64 μM. It can induce apoptosis and inhibit cell metastasis, making it a valuable compound for researching lung cancer [1].

Tubulin polymerization-IN-26 (compound 12h), at concentrations of 0.27–30 μM for 48 hours, demonstrates robust cytotoxicity against various cancer cell lines including lung, triple-negative breast, melanoma, and kidney cells by inducing apoptosis, causing G2/M phase cell cycle arrest, and inhibiting microtubule polymerization. At 0.1, 0.25, and 0.5 µM over 24 hours, it promotes dose-dependent apoptosis via enhanced ROS production, increasing the G2/M phase cell percentage from 17.6% to 50.3% while decreasing G0/G1 phase cells from 41.1% to 4.9%. The compound's cytotoxicity is quantified by IC50 values: 0.29 µM (A549), 1.48 µM (MDA-MB-231), 1.25 µM (B16-F10), 0.42 µM (BT-474), 0.49 µM (4 T1), and 1.58 µM (NRK-52E), underscoring its potential as an effective anti-cancer agent.