This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
Tubulin polymerization-IN-26
Catalog No. T61880 CAS
2490291-68-2
Tubulin polymerization-IN-26 (compound 12h) is a chemical compound that inhibits the polymerization of microtubulin. It achieves this by binding to the colchicine binding site of microtubulin, with an IC50 value of 4.64 μM. Additionally, Tubulin polymerization-IN-26 has the ability to induce apoptosis and inhibit cell metastasis or migration. Due to these properties, it holds potential as a valuable compound for researching lung cancer [1].
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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
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Biological Description
Chemical Properties
Storage
& Solubility Information
Description
Tubulin polymerization-IN-26 (compound 12h) is a chemical compound that inhibits the polymerization of microtubulin. It achieves this by binding to the colchicine binding site of microtubulin, with an IC50 value of 4.64 μM. Additionally, Tubulin polymerization-IN-26 has the ability to induce apoptosis and inhibit cell metastasis or migration. Due to these properties, it holds potential as a valuable compound for researching lung cancer [1].
In vitro
Tubulin polymerization-IN-26 (compound 12h), with concentrations ranging from 0.27-30 μM over 48 hours, demonstrates robust cytotoxic effects against various cancer cells, including lung, triple-negative breast, melanoma, and kidney cell lines, evidenced by apoptotic induction, cell cycle arrest in G2/M phase, and inhibition of microtubule protein polymerization. Notably, at concentrations of 0.1 µM, 0.25 µM, and 0.5 µM over 24 hours, it promotes dose-dependent apoptosis by enhancing cellular ROS production and significantly alters the cell cycle dynamics by increasing G2/M phase cell percentage (17.6% to 50.3%) while reducing G0/G1 phase cells (41.1% to 4.9%), corresponding to escalating concentrations. This compound’s pronounced cytotoxicity is quantified by varying IC50 values against the tested cell lines: 0.29 µM (A549), 1.48 µM (MDA-MB-231), 1.25 µM (B16-F10), 0.42 µM (BT-474), 0.49 µM (4 T1), and 1.58 µM (NRK-52E), showcasing its potential as an effective anti-cancer agent.
Molecular Weight
397.47
Formula
C25H23N3O2
CAS No.
2490291-68-2
Storage
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dose Conversion
You can also refer to dose conversion for different animals.
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Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.