Tris(4-aminophenyl)methane (Leucopararosaniline), a triphenylmethane dye, is a weak HCV helicase inhibitor.

Tris(4-aminophenyl)methane shows weak HCV helicase inhibition (30% inhibition at 100 μM) [1].

1. Use as an HCV helicase inhibitor
1. Dissolution: Leucopararosaniline is generally dissolved in DMSO or water, and the specific solvent depends on the experimental requirements. The solubility may be low, so an appropriate concentration needs to be used.
2. Inhibitor concentration: In experiments, the concentration of Leucopararosaniline is usually between 1-10 μM, and the specific concentration needs to be optimized according to experimental conditions.
3. Cell treatment: In the virus infection model, Leucopararosaniline is added to the cell culture and treated for a certain period of time to inhibit HCV helicase and observe its effect on viral replication.
2. Use as a dye:
1. Dissolve it in an appropriate solvent (such as PBS or DMSO), then add it to the experimental system for staining.
2. Specific staining conditions (such as staining concentration, time, etc.) need to be optimized according to experimental requirements.