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TN1

Name: TN1
Brand: TargetMol
SKU: T19583
Price: 97 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19583Cas No. 289479-94-3

TN1 is a potent inducer of fetal hemoglobin (HbF).

TN1

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Purity: 99.62%

Catalog No. T19583Cas No. 289479-94-3

TN1 is a potent inducer of fetal hemoglobin (HbF).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$97	In Stock	In Stock
2 mg	$142	In Stock	In Stock
5 mg	$239	In Stock	In Stock
10 mg	$347	-	In Stock
25 mg	$577	-	In Stock
50 mg	$823	-	In Stock
100 mg	$1,090	6-8 weeks	6-8 weeks
1 mL x 10 mM (in DMSO)	$268	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.62%

Appearance:Solid

Color:Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description

TN1 is a potent inducer of fetal hemoglobin (HbF).

In vitro

TN1 demonstrates superior efficacy in inducing fetal hemoglobin (HbF) in KU812 and K562 leukemia cell lines compared to hydroxyurea (HU), promoting HbF protein synthesis in a dose-dependent manner. At a concentration of 100 nM, TN1 significantly enhanced γ-globin expression—2.9-fold in KU812 cells and 3.7-fold in K562 cells—surpassing the enhancement achieved with 50-100 μM HU (1.8- and 1.9-fold increases, respectively), making it more effective than the standard SCD treatment. Remarkably, TN1's EC50 value for HbF induction is vastly lower than HU's (HU: EC50=50-100 μM; TN1: EC50=100 nM), highlighting its potency. Additionally, TN1 outperforms other small-molecule HbF inducers, including sodium butyrate and various histone deacetylase (HDAC) inhibitors. Its efficacy extends to increasing γ-globin mRNA transcription by more than fourfold, affecting both transcriptional and protein levels of γ-globin. Within 24 hours of treatment, an increase in both γ-globin mRNA and protein synthesis is observed. TN1 further triggers β-globin mRNA production alongside γ-globin mRNA, akin to the action of hydroxyurea, presenting a promising therapeutic candidate for further exploration.

Chemical Properties

Molecular Weight	509.6
Formula	C₂₉H₃₁N₇O₂
Cas No.	289479-94-3
Smiles	CCn1cnc2c(Nc3cccc(NC(=O)C#Cc4ccc(C)cc4)c3)nc(N[C@H]3CC[C@H](O)CC3)nc12
Relative Density.	1.31 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 90 mg/mL (176.61 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.48 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9623 mL	9.8116 mL	19.6232 mL	98.1162 mL
5 mM	0.3925 mL	1.9623 mL	3.9246 mL	19.6232 mL
10 mM	0.1962 mL	0.9812 mL	1.9623 mL	9.8116 mL
20 mM	0.0981 mL	0.4906 mL	0.9812 mL	4.9058 mL
50 mM	0.0392 mL	0.1962 mL	0.3925 mL	1.9623 mL
100 mM	0.0196 mL	0.0981 mL	0.1962 mL	0.9812 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc