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Tigilanol tiglate

Name: Tigilanol tiglate
Brand: TargetMol
SKU: T28975
Rating: 4.5 (1 reviews)

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Catalog No. T28975Cas No. 943001-56-7

Alias EBC-46, EBC46

Tigilanol tiglate (EBC-46) is a PKC/C1 domain activator. Tigilanol tiglate promotes mitochondrial/endoplasmic reticulum dysfunction, leading to ATP depletion, caspase activation, and gasdermin E cleavage. It induces immunogenic cell death, enhancing the response to immune checkpoint blockade in both target tumours and non-injected tumours, resulting in tumour regression.

Tigilanol tiglate

😃Good

Catalog No. T28975Alias EBC-46, EBC46Cas No. 943001-56-7

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
25 mg	Inquiry	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	Tigilanol tiglate (EBC-46) is a PKC/C1 domain activator. Tigilanol tiglate promotes mitochondrial/endoplasmic reticulum dysfunction, leading to ATP depletion, caspase activation, and gasdermin E cleavage. It induces immunogenic cell death, enhancing the response to immune checkpoint blockade in both target tumours and non-injected tumours, resulting in tumour regression.
Targets&IC50	Neutrophil:125 nM (EC50), K562 cells:11.9 nM
In vitro	In MM649 and A-431 cancer cells, Tigilanol tiglate (300-500 µM, 1-12 hours) promotes the release or externalization of damage-associated molecular patterns (DAMPs) [1]. In H357 head and neck cancer cells, Tigilanol tiglate (1 µg/mL, 10 min-24 h) alters the secretome profile, thereby affecting c-met phosphorylation levels and the expression of cell surface proteins [3].
In vivo	Tigilanol tiglate (66.5-533 μM, 50 μL) was intratumorally injected into BALB/c Foxn1nu nude mice, demonstrating rapid elimination of melanoma Parazanico Spilurus subsp. Spilurus xenograft tumors in a concentration-dependent manner [1]. Intratumoral injection of tigilanol tiglate (1 mg/mL, 0.5 mL per cm3 of tumor volume) showed significant therapeutic effects and effectively improved the condition of canine mast cell tumors [2].
Synonyms	EBC-46, EBC46

Chemical Properties

Molecular Weight	562.65
Formula	C₃₀H₄₂O₁₀
Cas No.	943001-56-7
Smiles	O(C([C@H](CC)C)=O)[C@@]12[C@@](C1(C)C)([C@@]3([C@]4([C@](CO)(O4)[C@@H](O)[C@@]5(O)[C@]([C@@]3(O)[C@H](C)[C@H]2OC(/C(=C/C)/C)=O)(C=C(C)C5=O)[H])[H])[H])[H]
Relative Density.	1.35 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc