This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
SHP2-IN-8
Catalog No. T61891 CAS
1801692-60-3
SHP2-IN-8 is a reversible, noncompetitive, selective and efficient allosteric SHP2 inhibitor (IC50=23 nM, Ki=22 nM), which also has effects in cells. SHP2-IN-8 can cause significant thermal displacement( Δ Tm = 7.01 ℃)。 SHP2-IN-8 can inhibit the phosphorylation of AKT and induce apoptosis of Hela cells.
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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
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Biological Description
Chemical Properties
Storage
& Solubility Information
Description
SHP2-IN-8 is a reversible, noncompetitive, selective and efficient allosteric SHP2 inhibitor (IC50=23 nM, Ki=22 nM), which also has effects in cells. SHP2-IN-8 can cause significant thermal displacement( Δ Tm = 7.01 ℃)。 SHP2-IN-8 can inhibit the phosphorylation of AKT and induce apoptosis of Hela cells.
In vitro
SHP2-IN-8 (compound TK-453), at concentrations ranging from 0 to 100 μM over a 48-hour period, exhibits moderate inhibitory effects on the proliferation of KYSE-70, Hela, and THP-1 cells. Additionally, when applied at 0 to 30 μM for 24 hours, SHP2-IN-8 prompts apoptosis in Hela cells in a dose-dependent manner. Furthermore, within a span of 0 to 2 hours and at concentrations of 0 to 30 μM, SHP2-IN-8 both concentration- and time-dependently hampers the phosphorylation of AKT in Hela and KYSE-70 cells. Through Cell Proliferation Assay, Apoptosis Analysis, and Western Blot Analysis, these effects were substantiated, identifying the compound's specific impact on cell growth dynamics, apoptosis induction, and AKT phosphorylation pathway inhibition within the tested cell lines [1].
Molecular Weight
398.35
Formula
C17H21Cl2N5S
CAS No.
1801692-60-3
Storage
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dose Conversion
You can also refer to dose conversion for different animals.
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Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.