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PPACK Dihydrochloride (Synonyms: Pebac dihydrochloride, D-Phe-Pro-Arg-CH2Cl 2HCl, D-Phenylalanyl-prolyl-arginy...)

Name: PPACK Dihydrochloride
Brand: TargetMol
SKU: T23177
Rating: 4.5 (1 reviews)

Catalog No. T23177 Copy Product Info

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PPACK Dihydrochloride is an efficient and selective irreversible thrombin inhibitor, which is often used in anticoagulant research, and can be used as a substitute anticoagulant for heparin lithium in blood gas and whole blood electrolyte analysis. PPACK is also used to regulate serine protease-related processes, which has application value in the study of thrombosis and fibrinolysis-related mechanisms.

PPACK Dihydrochloride

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Catalog No. T23177

Synonyms Pebac dihydrochloride, D-Phe-Pro-Arg-CH2Cl 2HCl, D-Phenylalanyl-prolyl-arginyl Chloromethyl Ketone dihydrochloride

Cas No. 82188-90-7

Pack Size	Price	USA Stock	Global Stock	Quantity
5 mg	$468	3-6 months	3-6 months

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	PPACK Dihydrochloride is an efficient and selective irreversible thrombin inhibitor, which is often used in anticoagulant research, and can be used as a substitute anticoagulant for heparin lithium in blood gas and whole blood electrolyte analysis. PPACK is also used to regulate serine protease-related processes, which has application value in the study of thrombosis and fibrinolysis-related mechanisms.
In vitro	PPACK Dihydrochloride can effectively inhibit thrombin, and its Ki value is 3.7 × 10-8 M[2]. PPACK Dihydrochloride (0.2 mM; 2 h coincubation, 12 h measurement) can reduce the permeability increase induced by plasmin in the confluent bovine aortic endothelial cell monolayer by 59% at 12 hours after the initial exposure of plasmin, and completely inhibit the activity of plasmin [3]. PPACK Dihydrochloride (0.2 mM; 4 h post plasmin treatment can alleviate the morphological damage of confluent bovine aortic endothelial cells induced by plasmin, including reducing intercellular space and maintaining the stress fiber structure of F-actin, which was detected after 4 hours of initial plasmin treatment [3].
In vivo	PPACK Dihydrochloride (0.05-0.5 mg/kg; i.v.; Single bolus injection) plays a significant and dose-dependent antithrombotic role in the rat mesenteric microthrombosis model induced by He-Ne laser. Its minimum effective dose to arterioles and venules is 0.1 mg/kg, and it reaches its maximum activity immediately after a single intravenous injection, and the activity can be maintained for 60 minutes[2].
Synonyms	Pebac dihydrochloride, D-Phe-Pro-Arg-CH2Cl 2HCl, D-Phenylalanyl-prolyl-arginyl Chloromethyl Ketone dihydrochloride

Chemical Properties

Molecular Weight	523.88
Formula	C₂₁H₃₃Cl₃N₆O₃
Cas No.	82188-90-7
Smiles	N=C(N)NCCC[C@@H](C(CCl)=O)NC([C@H]1N(CCC1)C([C@H](N)CC2=CC=CC=C2)=O)=O.Cl.Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100.00 mg/mL (190.88 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9088 mL	9.5442 mL	19.0883 mL	95.4417 mL
5 mM	0.3818 mL	1.9088 mL	3.8177 mL	19.0883 mL
10 mM	0.1909 mL	0.9544 mL	1.9088 mL	9.5442 mL
20 mM	0.0954 mL	0.4772 mL	0.9544 mL	4.7721 mL
50 mM	0.0382 mL	0.1909 mL	0.3818 mL	1.9088 mL
100 mM	0.0191 mL	0.0954 mL	0.1909 mL	0.9544 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc