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pNNP

Name: pNNP
Brand: TargetMol
SKU: T34093
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T34093Cas No. 330-13-2

Alias p-NNP, p-Nitrophenyl phosphate, p NNP, Nitrophenylphosphate

pNNP (Nitrophenylphosphate) can be used as a substrate for the PP2C assay. pNNP inhibits β-carbonic anhydrase and α-carbonate dehydrogenase, as well as H. pylori Helicobacter pylori. pNNP is a weakly active, tissue-nonspecific alkaline phosphatase modifier. pNNP has been shown to inhibit β-carbonic anhydrase and α-carbonate dehydrogenase.

pNNP

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Purity: 99.82%

Catalog No. T34093Alias p-NNP, p-Nitrophenyl phosphate, p NNP, NitrophenylphosphateCas No. 330-13-2

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$33	In Stock	In Stock
25 mg	$54	In Stock	In Stock
50 mg	$81	In Stock	In Stock
100 mg	$123	In Stock	In Stock
500 mg	$297	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.82%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

pNNP AI Summary

pNNP exhibits multiple bioactivities across different enzymes and biological targets. It demonstrates inhibitory activity against human serum paraoxonase (PON1) with an IC50 value of 18,500,000 nM and against the Src protein tyrosine kinase SH2 domain with an IC50 value of 1,000,000 nM. Additionally, pNNP acts as an inhibitor of CD45 protein tyrosine phosphatase, with kinetic parameters including a k cat of 3.95 s^-1, a Km of 278,000 nM, and a kcat/Km ratio of 0.000142 M^-1 s^-1. For carbonic anhydrase enzymes, pNNP shows varying catalytic efficiencies. It exhibits a high kcat/Km ratio for mouse carbonic anhydrase 13, indicating strong catalytic efficiency, whereas for human carbonic anhydrase 1 and 2, it demonstrates moderate and lower ratios, respectively. It also inhibits esterase activities of these enzymes as measured by the CO2 hydration method. Moreover, pNNP has inhibitory effects on several phosphatases, including Yersinia pestis PTPase (Km of 600,000 nM), and human STEP (Km of 745,000 nM). It also shows inhibitory activity against PtpB (Km of 1,336,000 nM) and DLODP in human HepG2 microsomal membranes, with an IC50 value of 4,200,000 nM. In terms of drug metabolism, pNNP is metabolized in mouse liver homogenates with significant hydrolysis at different pH levels, showing higher metabolism rates at pH 5 compared to pH 9.6. This indicates efficient liver metabolism under acidic conditions..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	pNNP (Nitrophenylphosphate) can be used as a substrate for the PP2C assay. pNNP inhibits β-carbonic anhydrase and α-carbonate dehydrogenase, as well as H. pylori Helicobacter pylori. pNNP is a weakly active, tissue-nonspecific alkaline phosphatase modifier. pNNP has been shown to inhibit β-carbonic anhydrase and α-carbonate dehydrogenase.
Synonyms	p-NNP, p-Nitrophenyl phosphate, p NNP, Nitrophenylphosphate

Chemical Properties

Molecular Weight	219.09
Formula	C₆H₆NO₆P
Cas No.	330-13-2
Smiles	O(P(=O)(O)O)C1=CC=C(N(=O)=O)C=C1
Relative Density.	1.712 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (273.86 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.5643 mL	22.8217 mL	45.6433 mL	228.2167 mL
5 mM	0.9129 mL	4.5643 mL	9.1287 mL	45.6433 mL
10 mM	0.4564 mL	2.2822 mL	4.5643 mL	22.8217 mL
20 mM	0.2282 mL	1.1411 mL	2.2822 mL	11.4108 mL
50 mM	0.0913 mL	0.4564 mL	0.9129 mL	4.5643 mL
100 mM	0.0456 mL	0.2282 mL	0.4564 mL	2.2822 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc