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L-NIO dihydrochloride

Name: L-NIO dihydrochloride
Brand: TargetMol
SKU: T11806
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T11806 Copy Product Info

Purity: 99.99%

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L-NIO dihydrochloride is a potent and selective nNOS inhibitor. It reduces NO production in the CNS to protect against oxidative damage, used for neuroinflammation and pain research.

L-NIO dihydrochloride

Copy Product Info

🥰Excellent

Catalog No. T11806

L-NIO dihydrochloride is a potent and selective nNOS inhibitor. It reduces NO production in the CNS to protect against oxidative damage, used for neuroinflammation and pain research.

Cas No. 159190-44-0

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Pack Size	Price	USA Stock	Global Stock
1 mg	$30	-	In Stock
5 mg	$56	-	In Stock
10 mg	$98	-	In Stock
25 mg	$162	-	In Stock
50 mg	$238	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.99%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	L-NIO dihydrochloride is a potent and selective nNOS inhibitor. It reduces NO production in the CNS to protect against oxidative damage, used for neuroinflammation and pain research.
Targets&IC50	iNOS:3.9 μM (Ki), eNOS:3.9 μM (Ki), nNOS:1.7 μM (Ki)
In vitro	L-NIO dihydrochloride exhibits NADPH-dependent NOS inhibition and causes irreversible inactivation as a mechanism-based suicide substrate [1].
In vivo	Striatal injection of L-NIO dihydrochloride (2.0 umol) induces focal cerebral ischemia in rats, serving as a standard agent for stroke models [2].

Chemical Properties

Molecular Weight	246.14
Formula	C₇H₁₇Cl₂N₃O₂
Cas No.	159190-44-0
Smiles	CC(NCCC[C@H](N)C(O)=O)=N.Cl.Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 33.33 mg/mL (135.41 mM), Sonication is recommended.

H2O: 100.00 mg/mL (406.27 mM), Sonication is recommmended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.00 mg/mL (8.13 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	4.0627 mL	20.3136 mL	40.6273 mL	203.1364 mL
5 mM	0.8125 mL	4.0627 mL	8.1255 mL	40.6273 mL
10 mM	0.4063 mL	2.0314 mL	4.0627 mL	20.3136 mL
20 mM	0.2031 mL	1.0157 mL	2.0314 mL	10.1568 mL
50 mM	0.0813 mL	0.4063 mL	0.8125 mL	4.0627 mL
100 mM	0.0406 mL	0.2031 mL	0.4063 mL	2.0314 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc