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KDOAM-25 citrate

Name: KDOAM-25 citrate
Brand: TargetMol
SKU: T11750
Rating: 4.5 (1 reviews)

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Catalog No. T11750Cas No. 2448475-08-7

KDOAM-25 citrate is a potent and highly selective histone lysine demethylases 5 (KDM5) inhibitor, with IC50 values of 71 nM, 19 nM, 69 nM, and 69 nM for KDM5A, KDM5B, KDM5C, and KDM5D, respectively. Multiple myeloma MM1S cells treated with KDOAM-25 citrate show increased global H3K4 methylation at transcriptional start sites and impaired proliferation [1].

KDOAM-25 citrate

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Catalog No. T11750Cas No. 2448475-08-7

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$1,820	10-14 weeks	10-14 weeks
50 mg	$2,380	10-14 weeks	10-14 weeks
100 mg	$3,100	10-14 weeks	10-14 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	KDOAM-25 citrate is a potent and highly selective histone lysine demethylases 5 (KDM5) inhibitor, with IC50 values of 71 nM, 19 nM, 69 nM, and 69 nM for KDM5A, KDM5B, KDM5C, and KDM5D, respectively. Multiple myeloma MM1S cells treated with KDOAM-25 citrate show increased global H3K4 methylation at transcriptional start sites and impaired proliferation [1].
Targets&IC50	KDM5C:69 nM , KDM5B:19 nM , KDM5D:69 nM , KDM5A:71 nM
In vitro	KDOAM-25 citrate inhibits most potently KDM5B with an IC 50 of ～50 μM and the other KDM5 family members at concentrations above 100 μM. KDOAM-25 citrate is inactive against any of the other tested JmjC family members [1]. KDOAM-25 citrate is able to reduce the viability of MM1S cells with an IC 50 of ～30 μM after a delay of 5-7 days [1]. KDOAM-25 citrate treatment results in a G1 cell-cycle arrest with an increased proportion of MM1S in G1 and a decrease of the proportion of cells in G2 without an increase in the proportion of cells in the apoptotic sub-G1 phase [1]. KDOAM-25 citrate (50 μM) increases with approximately twice as much H3K4me3 in in multiple myeloma cells [1].

Chemical Properties

Molecular Weight	499.51
Formula	C₂₁H₃₃N₅O₉
Cas No.	2448475-08-7
Smiles	NC(C1=CC=NC(CNCC(N(CC)CCN(C)C)=O)=C1)=O.OC(CC(C(O)=O)(O)CC(O)=O)=O
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 200 mg/mL (400.39 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 5 mg/mL (10.01 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0020 mL	10.0098 mL	20.0196 mL	100.0981 mL
5 mM	0.4004 mL	2.0020 mL	4.0039 mL	20.0196 mL
10 mM	0.2002 mL	1.0010 mL	2.0020 mL	10.0098 mL
20 mM	0.1001 mL	0.5005 mL	1.0010 mL	5.0049 mL
50 mM	0.0400 mL	0.2002 mL	0.4004 mL	2.0020 mL
100 mM	0.0200 mL	0.1001 mL	0.2002 mL	1.0010 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc