Indole-3-butyric acid potassium at a concentration of 10 μM induces the formation of adventitious roots (AR) in thin cell layers (TCL). Additionally, at a concentration of 1 μM, it promotes lateral root formation in Arabidopsis by facilitating the production of nitric oxide (NO).

For the direct quantification of 2-keto-L-gulonic acid (2-KLG) production on plates, three pH indicators were tested. Among them, Bromothymol Blue demonstrated superior performance due to its distinct color transition and appropriate pH range (blue to yellow at pH 6.5-7.5). When a solution of Bromothymol Blue (0.1%, w/v) is applied to an agar plate, the areas around colonies of K. vulgare 07 mutants shift from blue to yellow. This color change occurs because the K. vulgare 07 mutants secrete 2-KLG onto the agar, thus acidifying the immediate vicinity of the colonies.