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Daporinad hydrochloride (Synonyms: FK 866 hydrochloride (658084-64-1 free base), FK 866 hydrochloride, APO866)

Name: Daporinad hydrochloride
Brand: TargetMol
SKU: T22785
Rating: 4.5 (1 reviews)

Catalog No. T22785 Copy Product Info

Purity: 98.99%

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Daporinad hydrochloride (FK 866 hydrochloride) is a potent nicotinamide-phosphate ribosyltransferase (NAMPT) inhibitor with potential anti-tumor and anti-angiogenic activity that induces apoptosis in tumor cells.Daporinad hydrochloride can be used in the study of inflammation and cancer.

Daporinad hydrochloride

Copy Product Info

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Catalog No. T22785

Synonyms FK 866 hydrochloride (658084-64-1 free base), FK 866 hydrochloride, APO866

Cas No. 1785666-54-7

Pack Size	Price	USA Stock	Global Stock	Quantity
1 mg	$30	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$78	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.99%

Appearance:Oil

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Daporinad hydrochloride (FK 866 hydrochloride) is a potent nicotinamide-phosphate ribosyltransferase (NAMPT) inhibitor with potential anti-tumor and anti-angiogenic activity that induces apoptosis in tumor cells.Daporinad hydrochloride can be used in the study of inflammation and cancer.
In vitro	METHODS: RPMI8226/S, U266 and MM1S cells were treated with FK866 (10nM, 24-48 hours) to study the effect of Nampt inhibitor on MAPK signaling pathway in MM cells. RESULTS: FK866 significantly reduced the gene and protein levels of pERK1/2, pp38MAPK and pSAPK/JNK, indicating that the drug has an effective inhibitory effect on MEK/ERK signaling. [1]
In vivo	METHODS: CB17-SCID mice injected with MM1S cells were treated with FK866 (30 mg/kg, intraperitoneal injection, twice a day, for 4 days) once a week for 3 weeks. Caliper measurements of the longest perpendicular tumor diameter were taken twice a week, and excised tumors were collected from mice for Western blot analysis to assess ERK and LC3B phosphorylation. RESULTS: A significant reduction in tumor burden was observed on day 7 of FK866 treatment, the treatment was well tolerated, without significant weight loss or neurological changes, and resulted in a significant prolongation of overall survival; tumor tissues from FK866-treated mice showed a significant reduction in ERK phosphorylation and LC3 proteolytic cleavage. [1]
Synonyms	FK 866 hydrochloride (658084-64-1 free base), FK 866 hydrochloride, APO866

Chemical Properties

Molecular Weight	427.97
Formula	C₂₄H₃₀ClN₃O₂
Cas No.	1785666-54-7
Smiles	Cl.O=C(C=CC=1C=NC=CC1)NCCCCC2CCN(C(=O)C=3C=CC=CC3)CC2
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 10 mg/mL (23.37 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3366 mL	11.6831 mL	23.3661 mL	116.8306 mL
5 mM	0.4673 mL	2.3366 mL	4.6732 mL	23.3661 mL
10 mM	0.2337 mL	1.1683 mL	2.3366 mL	11.6831 mL
20 mM	0.1168 mL	0.5842 mL	1.1683 mL	5.8415 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc