EGFR/BRAFV600E-IN-4 (Compound 10f) is a dual inhibitor of EGFR and BRAFV600E, with IC50 values of 61 nM and 43 nM, respectively. It halts the cell cycle, induces apoptosis in both early and late stages, and inhibits cancer cell growth in vitro, showing broad-spectrum anticancer activity.

EGFR:61 nM

EGFR/BRAFV600E-IN-4 (0.01-100 μM) demonstrates broad-spectrum antiproliferative activity with GI50 values ranging from 1.66 to greater than 100 μM. It effectively inhibits the growth of A-549, Panc-1, HT-29, and MCF-7 cells with IC50 values between 24 nM and 27 nM over 48 hours (1.56-100 nM), while showing relative safety toward MCF-10A cells, with a cell viability of 91%. At a concentration of 24 nM for 24 hours, EGFR/BRAFV600E-IN-4 induces cell cycle arrest in the G0/G1 phase, hindering the transition from G1 to S and G2/M phases, and triggers apoptosis both in the early and late stages.