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Dihydrofluorescein diacetate

Name: Dihydrofluorescein diacetate
Brand: TargetMol
SKU: T18976
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18976Cas No. 35340-49-9

Alias Diacetyldihydrofluorescein

Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.

Dihydrofluorescein diacetate

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Purity: 99.98%

Catalog No. T18976Alias DiacetyldihydrofluoresceinCas No. 35340-49-9

Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
50 mg	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.98%

Appearance:Solid

Color:Orange

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.
In vitro	Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence sharply delineates filaments that correspond in all detail to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix[1]. Dihydrofluorescein diacetate may be a useful quantitative method to measure the oxidation potential of nanoparticle-treated cells[2]. Dihydrofluorescein diacetate shows fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium[3].
Cell Research	Dihydrofluorescein diacetate to measure intracellular ROS levels Operation steps 1. Cell culture: Cultivate cells in a normal way until they are in a suitable state. 2. Dye treatment: Add 20 μM DHF for 30 minutes. 3. Rinse with PBS to remove residual dye. 4. Observe the fluorescence intensity under a microscope to determine the ROS level in mitochondria. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Diacetyldihydrofluorescein

Chemical Properties

Molecular Weight	418.4
Formula	C₂₄H₁₈O₇
Cas No.	35340-49-9
Smiles	CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c1ccccc1C(O)=O
Relative Density.	1.353g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (239.01 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3901 mL	11.9503 mL	23.9006 mL	119.5029 mL
5 mM	0.4780 mL	2.3901 mL	4.7801 mL	23.9006 mL
10 mM	0.2390 mL	1.1950 mL	2.3901 mL	11.9503 mL
20 mM	0.1195 mL	0.5975 mL	1.1950 mL	5.9751 mL
50 mM	0.0478 mL	0.2390 mL	0.4780 mL	2.3901 mL
100 mM	0.0239 mL	0.1195 mL	0.2390 mL	1.1950 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitDihydrofluorescein diacetateDihydrofluorescein