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Dihydrofluorescein diacetate

Name: Dihydrofluorescein diacetate
Brand: TargetMol
SKU: T18976
Rating: 4.5 (1 reviews)

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Catalog No. T18976Cas No. 35340-49-9

Alias Diacetyldihydrofluorescein

Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.

Dihydrofluorescein diacetate

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Purity: 98.52%

Catalog No. T18976Alias DiacetyldihydrofluoresceinCas No. 35340-49-9

Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.

Pack Size	Price	Availability	Quantity
50 mg	$29	Backorder
1 mL x 10 mM (in DMSO)	$29	Backorder

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Batch Information

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Purity:98.52%

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.
In vitro	Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence sharply delineates filaments that correspond in all detail to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix[1]. Dihydrofluorescein diacetate may be a useful quantitative method to measure the oxidation potential of nanoparticle-treated cells[2]. Dihydrofluorescein diacetate shows fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium[3].
Cell Research	Dihydrofluorescein diacetate to measure intracellular ROS levels Operation steps 1. Cell culture: Cultivate cells in a normal way until they are in a suitable state. 2. Dye treatment: Add 20 μM DHF for 30 minutes. 3. Rinse with PBS to remove residual dye. 4. Observe the fluorescence intensity under a microscope to determine the ROS level in mitochondria.
Alias	Diacetyldihydrofluorescein

Chemical Properties

Molecular Weight	418.4
Formula	C₂₄H₁₈O₇
Cas No.	35340-49-9
Smiles	CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c1ccccc1C(O)=O
Relative Density.	1.353g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (239.01 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3901 mL	11.9503 mL	23.9006 mL	119.5029 mL
5 mM	0.4780 mL	2.3901 mL	4.7801 mL	23.9006 mL
10 mM	0.2390 mL	1.1950 mL	2.3901 mL	11.9503 mL
20 mM	0.1195 mL	0.5975 mL	1.1950 mL	5.9751 mL
50 mM	0.0478 mL	0.2390 mL	0.4780 mL	2.3901 mL
100 mM	0.0239 mL	0.1195 mL	0.2390 mL	1.1950 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitDihydrofluorescein diacetateDihydrofluorescein DiacetateDihydrofluorescein