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Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 mg | $29 | Backorder | |
1 mL x 10 mM (in DMSO) | $29 | Backorder |
Description | Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models. |
In vitro | Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence sharply delineates filaments that correspond in all detail to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix[1]. Dihydrofluorescein diacetate may be a useful quantitative method to measure the oxidation potential of nanoparticle-treated cells[2]. Dihydrofluorescein diacetate shows fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium[3]. |
Cell Research | Dihydrofluorescein diacetate to measure intracellular ROS levels Operation steps 1. Cell culture: Cultivate cells in a normal way until they are in a suitable state. 2. Dye treatment: Add 20 μM DHF for 30 minutes. 3. Rinse with PBS to remove residual dye. 4. Observe the fluorescence intensity under a microscope to determine the ROS level in mitochondria. |
Alias | Diacetyldihydrofluorescein |
Molecular Weight | 418.4 |
Formula | C24H18O7 |
Cas No. | 35340-49-9 |
Smiles | CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c1ccccc1C(O)=O |
Relative Density. | 1.353g/cm3 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
Solubility Information | DMSO: 100 mg/mL (239.01 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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