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DB1976 dihydrochloride

Name: DB1976 dihydrochloride
Brand: TargetMol
SKU: T10964L
Rating: 4.5 (1 reviews)

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Catalog No. T10964LCas No. 2369663-93-2

Alias DB1976 hydrochloride, DB1976 2HCl

DB1976 dihydrochloride (DB1976 2HCl) , a transcription factor PU.1 inhibitor with potential anti-inflammatory activity, slowed down inflammation and fibrosis and improved glucose homeostasis and dyslipidemia in mice in in vivo experiments.DB1976 dihydrochloride promotes apoptosis and may be used in studies of metabolic dysfunction and non-alcoholic steatohepatitis.

DB1976 dihydrochloride

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Purity: 99.08%

Catalog No. T10964LAlias DB1976 hydrochloride, DB1976 2HClCas No. 2369663-93-2

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$390	1-2 weeks	1-2 weeks
25 mg	$1,330	1-2 weeks	1-2 weeks
50 mg	$1,740	1-2 weeks	1-2 weeks
100 mg	$2,500	1-2 weeks	1-2 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.08%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	DB1976 dihydrochloride (DB1976 2HCl) , a transcription factor PU.1 inhibitor with potential anti-inflammatory activity, slowed down inflammation and fibrosis and improved glucose homeostasis and dyslipidemia in mice in in vivo experiments.DB1976 dihydrochloride promotes apoptosis and may be used in studies of metabolic dysfunction and non-alcoholic steatohepatitis.
Targets&IC50	Hematopoietic cells:334 μM, PU.1-negative HEK293 cells:2.4 μM, PU.1:10 nM, PU.1 URE–/–AML cells:105 μM
In vitro	DB1976 dihydrochloride , a classic heterocyclic dication with strong affinity and selectivity for AT-rich sequences commonly found in cognate DNA binding sites for PU.1[3], inhibits PU.1-dependent transactivation of the reporter in a dose-dependent manner with an IC50 value of 2.4 μM in PU.1-negative HEK293 cells[2]. Treatment with DB1976 dihydrochloride leads to a profound decrease in the growth of PU.1 URE–/– AML cells (IC50 of 105 μM), while exhibiting little effect on normal hematopoietic cells at similar concentrations (IC50 of 334 μM)[2].Furthermore,DB1976 dihydrochloride treatment results in a 1.6-fold increase in apoptotic cells in murine PU.1 URE–/– AML cells, with similar effects observed in human MOLM13 cells[3].In addition, DB1976 dihydrochloride treatment causes a significant decrease in the number of viable cells (primary human AML cells) (mean decrease of 81%) and clonogenic capacity (mean decrease of 36%) compared to vehicle-treated cells. The apoptotic cell fraction increases on average by 1.5-fold with DB1976 dihydrochloride[2].
Synonyms	DB1976 hydrochloride, DB1976 2HCl

Chemical Properties

Molecular Weight	520.28
Formula	C₂₀H₁₈Cl₂N₈Se
Cas No.	2369663-93-2
Smiles	N=C(C1=CC=C2N=C(C3=CC=C(C4=NC5=C(N4)C=C(C(N)=N)C=C5)[Se]3)NC2=C1)N.Cl.Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 20 mg/mL (38.44 mM), Sonication is recommended.

H2O: 10 mg/mL (19.22 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (3.84 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9220 mL	9.6102 mL	19.2204 mL	96.1021 mL
5 mM	0.3844 mL	1.9220 mL	3.8441 mL	19.2204 mL
10 mM	0.1922 mL	0.9610 mL	1.9220 mL	9.6102 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.0961 mL	0.4805 mL	0.9610 mL	4.8051 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc