CRT0063465 is a ligand for both human Phosphoglycerate Kinase 1 (PGK1) and the stress sensor protein DJ-1, exhibiting a dissociation constant (Kd) of 24 μM for PGK1. Additionally, it influences the composition of the shelterin complex and modulates telomere length [1].

CRT0063465, at concentrations of 10 and 100 nM for up to 84 days, inhibits telomere shortening in HCT116 cells under hypoglycemic conditions [1]. At 10 nM for one week, CRT0063465 modulates the composition of shelterin complexes in HCT116 cells under non-stress conditions [1]. Furthermore, at 100 nM for up to 100 hours, CRT0063465 provides cytoprotective effects against Bleomycin-induced toxicity in A2780 cells [1].