CMP-5 hydrochloride

Name: CMP-5 hydrochloride
Brand: TargetMol
SKU: T19243
Rating: 4.5 (1 reviews)

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Catalog No. T19243Cas No. 1030021-40-9

CMP-5 hydrochloride is a potent and selective PRMT5 inhibitor, while displays no activity against PRMT1/4/7 enzymes. It selectively blocks S2Me-H4R3 by inhibiting PRMT5 methyltransferase activity on histone preparations.

CMP-5 hydrochloride

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Catalog No. T19243Cas No. 1030021-40-9

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$1,520	1-2 weeks	1-2 weeks
50 mg	$1,980	1-2 weeks	1-2 weeks
100 mg	$2,500	1-2 weeks	1-2 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	CMP-5 hydrochloride is a potent and selective PRMT5 inhibitor, while displays no activity against PRMT1/4/7 enzymes. It selectively blocks S2Me-H4R3 by inhibiting PRMT5 methyltransferase activity on histone preparations.
Targets&IC50	MTH1 (cells):3.7 μM , Th2 cells (human):36.1 μM , MTH2 (cells):9.2 μM , Th1 cells (human):26.9 μM
In vitro	CMP-5 (0-100 μM; 24-72 hours) exhibits selective toxicity towards lymphoma cells, while showing limited toxicity to normal resting B lymphocytes, even with extended incubation. CMP-5 (0-40 μM; 24 hours) preferentially inhibits the proliferation of human Th1 cells over Th2 cells (43% vs. 9% inhibition, respectively), with IC50 values of 26.9 μM for Th1 cells and 31.6 μM for Th2 cells, indicating differential sensitivity to PRMT5 inhibition. CMP-5 (40 μM; 24 hours) reduces p-BTK and pY(416)SRC expression in 60A cells compared to the DMSO-treated group. Additionally, CMP-5 (25 μM; 24 hours) alone inhibits mouse Th1 cell proliferation by 91%; however, the addition of varying doses of IL-2 enhances proliferation, peaking at 5 ng/ml [1].

Chemical Properties

Molecular Weight	351.87
Formula	C₂₁H₂₂ClN₃
Cas No.	1030021-40-9
Smiles	Cl.N=1C=CC=CC1CNCC2=CC=C3C(=C2)C=4C=CC=CC4N3CC
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc