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CKI-7 free base

Name: CKI-7 free base
Brand: TargetMol
SKU: T10829
Rating: 4.5 (1 reviews)

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Catalog No. T10829Cas No. 120615-25-0

Alias CKI-7

CKI-7 free base is a potent, ATP-competitive inhibitor of casein kinase 1 (CK1; IC50: 6 μM; Ki: 8.5 μM) and a selective Cdc7 kinase inhibitor, also inhibiting SGK, ribosomal S6 kinase-1 (S6K1), and MSK1.

CKI-7 free base

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Catalog No. T10829Alias CKI-7Cas No. 120615-25-0

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
2 mg	$88	5 days	5 days
1 mL x 10 mM (in DMSO)	$128	5 days	5 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	CKI-7 free base is a potent, ATP-competitive inhibitor of casein kinase 1 (CK1; IC50: 6 μM; Ki: 8.5 μM) and a selective Cdc7 kinase inhibitor, also inhibiting SGK, ribosomal S6 kinase-1 (S6K1), and MSK1.
Targets&IC50	CK1:6 μM, CK1:8.5 μM (ki)
In vitro	The treatment of CKI-7 (0.1-10 μM; 5 days; ES cells) significantly increases the expression of the early neuroectodermal marker Sox1 and the number of cells positive for the neural markers βIII-tubulin and nestin, in a concentration-dependent manner. CKI-7 (5 μM; 5 days; ES cells) treatment suppresses SFEB-induced β-catenin stabilization on day 5 [1].
In vivo	In vivo dose-dependent, the anti-tumor activity of CKI-7 is demonstrated in a SCID-Beige mouse systemic tumor model utilizing isolated Philadelphia chromosome-positive acute lymphoblastic leukemia cell line. Exposure to CKI-7 results in a cell cycle-dependent caspase 3 activation and apoptotic cell death [2].
Synonyms	CKI-7

Chemical Properties

Molecular Weight	285.75
Formula	C₁₁H₁₂ClN₃O₂S
Cas No.	120615-25-0
Smiles	NCCNS(=O)(=O)c1ccc(Cl)c2ccncc12
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 25 mg/mL (87.49 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (7 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4996 mL	17.4978 mL	34.9956 mL	174.9781 mL
5 mM	0.6999 mL	3.4996 mL	6.9991 mL	34.9956 mL
10 mM	0.3500 mL	1.7498 mL	3.4996 mL	17.4978 mL
20 mM	0.1750 mL	0.8749 mL	1.7498 mL	8.7489 mL
50 mM	0.0700 mL	0.3500 mL	0.6999 mL	3.4996 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc