Antibacterialagent 207 (Compound Ru1) exhibits antibacterial activity against Staphylococcus aureus with a MIC of 1 μg/mL and a low resistance frequency. It disrupts bacterial cell membranes and promotes the production of reactive oxygen species (ROS) within the bacteria.

Antibacterial agent 207 (1 and 2 μg/mL) causes damage to the bacterial membrane of Staphylococcus aureus, indicated by PI staining, and triggers the release of β-galactosidase. At concentrations of 1 and 2 μg/mL for 2 hours, it induces various oxidative stress damages and ROS production in Staphylococcus aureus. Additionally, at 0.25 or 0.5 μg/mL over 48 hours, this agent inhibits α-toxin secretion and the formation of bacterial biofilms in Staphylococcus aureus.

Antibacterial agent 207, when administered at a dose of 5 mg/kg and 5 μL via right abdominal foot injection, demonstrates anti-infective properties and enhances the survival rate of Galleria mellonella infected with Staphylococcus aureus. Additionally, Antibacterial agent 207, applied at 100 μg/mL to wounds twice daily, exhibits strong anti-infective efficacy against Staphylococcus aureus on the skin of mice.