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SMCC

Catalog No. T9116 CAS 64987-85-5

Synonyms: N-Succinimidyl 4-(N-maleimidomethyl)cycl

SMCC (N-Succinimidyl 4-(N-maleimidomethyl)cycl) is a heterobifunctional protein crosslinker.

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SMCC, CAS 64987-85-5

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50 mg	In stock	$ 30.00
100 mg	In stock	$ 45.00
1 mL * 10 mM (in DMSO)	In stock	$ 45.00

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Purity: 98.96%

Biological Description

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Storage & Solubility Information

Description	SMCC (N-Succinimidyl 4-(N-maleimidomethyl)cycl) is a heterobifunctional protein crosslinker.
In vitro	N-Succinimidyl 4-(N-maleimidomethyl)cycl（SMCC) is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks.
Cell Research	Branched PEI (25 kDa) was dissolved in PBS buffer to yield a concentration of 1 mg/mL. No-weigh Sulfo-SMCC was dissolved in Milli-Q water with a concentration of 10 mg/mL, then a certain of the prepared no-weigh SulfoSMCC solution was added into 2 mL PEI solution to incubate for 30 min at 37 C. After the PEI-SMCC polymer was formed, TAT solution was added into the polymer at the given molar ratio and incubated for 2 h at 4 C. The concentration of unreacted SMCC and unreacted TAT were removed using Zeba Spin Desalting Columns. The polymer PEI-SMCC-TAT was also characterized and analyzed through FTIR to check if each element was added to the PEI backbone.

Synonyms	N-Succinimidyl 4-(N-maleimidomethyl)cycl
Molecular Weight	334.32
Formula	C16H18N2O6
CAS No.	64987-85-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 25 mg/mL (74.78 mM)

References and Literature

1. Li F, Wang Z, Huang Y, Xu H, He L, Deng Yan, Zeng X, He N. Delivery of PUMA Apoptosis Gene Using Polyethyleneimine-SMCC-TAT/DNA Nanoparticles: Biophysical Characterization and In Vitro Transfection Into Malignant Melanoma Cells. J Biomed Nanotechnol. 2015 Oct;11(10):1776-82. PubMed PMID: 26502640.

Related compound libraries

This product is contained In the following compound libraries：

NO PAINS Compound Library Bioactive Compound Library Immunology/Inflammation Compound Library Bioactive Compounds Library Max

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SMCC 64987-85-5 Others N-Succinimidyl 4-(N-maleimidomethyl)cycl ADC Linkers inhibit Antibody-drug conjugates linkers Inhibitor inhibitor

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