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Bromophenol Blue, an acid phthalein dye, is utilized both as a tracking dye for electrophoresis and as a pH indicator with a transition range of pH 3 to 4.6.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 g | $29 | In Stock | |
1 mL x 10 mM (in DMSO) | $29 | In Stock |
Description | Bromophenol Blue, an acid phthalein dye, is utilized both as a tracking dye for electrophoresis and as a pH indicator with a transition range of pH 3 to 4.6. |
In vitro | Instructions 1. Solvent selection: Bromophenol blue is usually dissolved in water, but can also be dissolved in organic solvents such as ethanol or DMSO depending on the specific experimental needs. 2. Electrophoresis tracking: Bromophenol blue is often used as a tracking dye in gel electrophoresis. It migrates with the sample and helps monitor the progress of the electrophoretic separation process. Concentration: Typical concentrations are 0.01% to 0.1% (w/v). Directions: The dye is mixed with the sample and loaded into the gel. 3. pH indicator: Bromophenol blue is a pH indicator that can change in the pH range of 3.0 to 4.6. It is yellow at low pH and blue at high pH. Directions: Prepare a diluted Bromophenol blue solution for monitoring pH changes in buffers or solutions. Protein detection: Bromophenol blue can be used to detect proteins by binding to the protein, which causes a color change that can be detected by spectrophotometry. 4. Absorption peak: When bound to proteins, Bromophenol blue produces a strong absorption peak at 610 nm. Sensors for chemical detection: Bromophenol blue can also be used as a sensor to detect a variety of compounds such as ammonia, drugs, proteins, and amino acids because it undergoes a color change when bound to these substances. |
Molecular Weight | 669.96 |
Formula | C19H10Br4O5S |
Cas No. | 115-39-9 |
Smiles | O=S1(=O)OC(C=2C1=CC=CC2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC(Br)=C(O)C(Br)=C4 |
Relative Density. | 0.954 g/cm3 at 20℃ |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | |||||||||||||||||||||||||||||||||||
Solubility Information | H2O: 11 mg/mL (16.42 mM), Sonication is recommended. DMSO: 55 mg/mL (82.09 mM), Sonication is recommended. ![]() | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
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