2-Thenoyltrifluoroacetone (TTFA) is a specific inhibitor of mitochondrial respiratory chain complex II (SQR/SDH) with an IC₅₀ value of 51.5 μM. 2-Thenoyltrifluoroacetone can complex with various metal ions and exhibits cytotoxic and antitumor activity. It possesses anti-Mycobacterium tuberculosis activity and can inhibit the growth of Mycobacterium tuberculosis. 2-Thenoyltrifluoroacetone can be used in mechanistic studies of diseases associated with mitochondrial dysfunction.

Mitochondrial respiratory chain complex II/SDH/SQR:51.5 μM

Methods: RTH-149 rainbow trout liver cell lines stably transfected with KFERQ-PA-mCherry1 were treated with 10 μM 2-Thenoyltrifluoroacetone and 25 mM high glucose for 16 hours, and CMA puncta were counted using fluorescence microscopy.
Results: Treatment with 2-thenoil-trifluoroacetone blocked the activity of complex II of the mitochondrial electron transport chain and prevented high-glucose-induced CMA puncta formation. [1]
Methods: TET21N, SK-N-BE(2), SH-SY5Y neuroblastoma cells, and U1810 lung cancer cells were treated with 100 μM 2-thienoyltrifluoroacetone and 5 μg/mL cisplatin for 24 hours, and Western blot analysis was performed to detect the levels of cleaved caspase-3 and cleaved PARP.
Results: Under normoxic conditions, 2-Thenoyltrifluoroacetone significantly enhanced cisplatin-induced apoptosis, with a significant increase in the expression of caspase-3 and cleaved PARP. [2]
Methods: PC12 rat pheochromocytoma cells were treated with 10 μM 2-Thenoyltrifluoroacetone and 1 μM rotenone for 24 hours, and intracellular H₂O₂ levels were detected using H₂DCFDA fluorescence staining.
Results: 2-Thienoyltrifluoroacetone significantly reduced the H₂O₂ production induced by rotenone. [3]

DMSO: 80 mg/mL (360.07 mM), Sonication is recommended.