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MCP-1/CCL2 Protein, Mouse, Recombinant (His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is P10148.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 μg | $137 | 7-10 days | 7-10 days | |
| 10 μg | $230 | 7-10 days | 7-10 days | |
| 20 μg | $383 | 7-10 days | 7-10 days | |
| 50 μg | $629 | 7-10 days | 7-10 days | |
| 100 μg | $1,050 | 7-10 days | 7-10 days |
| Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | MCP-1/CCL2 Protein, Mouse, Recombinant (His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is P10148. |
| Species | Mouse |
| Expression System | Baculovirus Insect Cells |
| Tag | C-His |
| Accession Number | P10148 |
| Synonyms | SMC-CF,Sigje,Scya2,MCP-1,MCP1,MCAF,JE,HC11,chemokine (C-C motif) ligand 2,AI323594 |
| Construction | A DNA sequence encoding the mouse CCL2 (NP_035463.1) (Met1-Asn148) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro. Predicted N terminal: Gln 24 |
| Protein Purity | > 80 % as determined by SDS-PAGE |
| Molecular Weight | 15.2 kDa (predicted); 27 kDa (reducing conditions) |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, Lyophilized powders are shipping with blue ice. |
| Research Background | Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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