CD3D Protein, Mouse, Recombinant (hFc)

Name: CD3D Protein, Mouse, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-03073
Rating: 4.5 (1 reviews)

(Synonyms: T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)) Copy Product Info

Synonyms: T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)

Catalog No. TMPY-03073 Copy Product Info

CD3D Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 36.4 kDa and the accession number is P04235.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days
200 µg	$1,230	7-10 days	7-10 days
500 µg	$2,530	7-10 days	7-10 days
1 mg	$5,300	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD3D Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 36.4 kDa and the accession number is P04235.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P04235
Construction	A DNA sequence encoding the mouse CD3D (P04235) (Met1-Ala105) was expressed, fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Phe 22
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
Research Background	T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCR alpha and beta chains along with invariant CD3 gamma, delta, epsilon, and zeta chains. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3 delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCR gamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

36.4 kDa (predicted); 44-48 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.