WRN inhibitor 6 (compound 3ci) is an effective inhibitor of WRN, inducing apoptosis and increasing the expression of p-p53, P-Chk2, and γH2AX.

WRN inhibitor 6 (compound 3ci) induces DNA damage and apoptosis in HCT116 and SW620 cells, with IC50 values of 2.305 µM and 16.58 µM, respectively, when used at concentrations of 0-100 µM for 72 hours. Additionally, WRN inhibitor 6 at concentrations of 0, 2.5, 5, and 10 µM over 3 and 27 hours increases the expression of p-p53, P-Chk2, and γH2AX in a dose-dependent manner in HCT116 cells.

WRN inhibitor 6 has low oral bioavailability in mice, recorded at 11.43%, and a short half-life of 0.17 hours when administered intravenously (20 mg/kg for p.o.; 5 mg/kg for i.v.).