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Tubulin polymerization-IN-4

Name: Tubulin polymerization-IN-4
Brand: TargetMol
SKU: T61940
Rating: 4.5 (1 reviews)

Catalog No. T61940 Copy Product Info

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Tubulin polymerization-IN-4 is an effective inhibitor of tubulin polymerization, and its IC₅₀ is 4.6 μM m. In HeLa cells, Tubulin polymerization-IN-4 can interfere with microtubule assembly and destroy the intracellular microtubule network, leading to cell cycle stagnation in G2/M phase, inducing cell apoptosis, and inhibiting cell clone formation and migration ability.

Tubulin polymerization-IN-4

Copy Product Info

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Catalog No. T61940

Cas No. 2835559-00-5

Pack Size	Price	USA Stock	Global Stock
25 mg	$1,520	10-14 weeks	10-14 weeks
50 mg	$1,980	10-14 weeks	10-14 weeks
100 mg	$2,500	10-14 weeks	10-14 weeks

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description	Tubulin polymerization-IN-4 is an effective inhibitor of tubulin polymerization, and its IC₅₀ is 4.6 μM m. In HeLa cells, Tubulin polymerization-IN-4 can interfere with microtubule assembly and destroy the intracellular microtubule network, leading to cell cycle stagnation in G2/M phase, inducing cell apoptosis, and inhibiting cell clone formation and migration ability.
Targets&IC50	HeLa cells:0.09 μM, SiHa cells:0.15 μM, HK-2 cells:188 μM, Tubulin:4.6 μM
In vitro	Tubulin polymerization-IN-4 (compound 9j) (0-1 μM; 48 hours) exhibits sub-micromolar inhibitory activities against HeLa, SiHa and MS751 [1]. Tubulin polymerization-IN-4 (3, 6 and 12.5 μM; 0-20 min) inhibits tubulin polymerization in a concentration-dependent manner with the inhibition percentages of 39%, 54%, and 77% at 3, 6 and 12.5 μM [1]. Tubulin polymerization-IN-4 (1-100 μM; 2 hours) inhibits the formation of EBI-β-tubulin adduct in a concentration-dependent manner [1]. Tubulin polymerization-IN-4 (0.2 μM; 1 and 2 hours) disrupts the HUVEC-formed vascular tube [1]. Tubulin polymerization-IN-4 (0.1-0.4 μM; 24 hours) increases cell distribution to the G2/M phase in a concentration-dependent manner [1]. Tubulin polymerization-IN-4 (0.1-0.4 μM; 24 hours) induces apoptosis of HeLa cells [1]. Tubulin polymerization-IN-4 (20, 50, 100 nM; 14 days) reduces new colony formation and suppresses HeLa cell growth for 14 days in a dose-dependent manner [1]. Tubulin polymerization-IN-4 (0.1, 0.2 and 0.4 μM; 24 hours) effectively inhibits the migration of HeLa cells in a concentration-dependent manner [1]. Tubulin polymerization-IN-4 (0-200 μM; 24 hours) exhibits good renal safety profile, with IC 50 of 188 ± 16 μM in HK-2 cells [1].
In vivo	Tubulin polymerization-IN-4, administered intraperitoneally (IP) at doses ranging from 100 to 1000 mg/kg, exhibits very low toxicity, with its lethal dose 50 (LD50) exceeding 1000 mg/kg [1]. When given daily for 21 days at doses of 30 and 60 mg/kg, it effectively inhibits tumor growth, achieving tumor growth inhibition (TGI) rates of 35% and 58%, respectively, at these doses [1].

Chemical Properties

Molecular Weight	400.86
Formula	C₂₁H₂₁ClN₂O₄
Cas No.	2835559-00-5
Smiles	ClC1=CC(=CC=C1OCC)C2=CN=CN=C2C=3C=C(OC)C(OC)=C(OC)C3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc