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Topoisomerase I/II inhibitor 2

Catalog No. T61045

Topoisomerase I/II inhibitor 2 (compound 1a) can significantly reduce the xenograft tumor growth in mice model which can be used in treating liver cancer. Topoisomerase I/II inhibitor 2 is a DNA topoisomerase I/II dual inhibitor. Topoisomerase I/II inhibitor 2 is a potent Topoisomerase inhibitor with IC50 values of 6.83 μM and 9.82 μM for LM9 cells and Huh7 cells [1].

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Topoisomerase I/II inhibitor 2 Chemical Structure

Topoisomerase I/II inhibitor 2, CAS N/A

Pack Size	Availability	Price/USD
25 mg	10-14 weeks	$ 1,520.00
50 mg	10-14 weeks	$ 1,980.00
100 mg	10-14 weeks	$ 2,500.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

Topoisomerase I/II inhibitor 2 (compound 1a) demonstrates a range of beneficial activities against cancer cell lines, showing strong anti-proliferative effects at concentrations between 0-150 μM over 72 hours, particularly against two human hepatocellular carcinoma cell lines, HuH7 and LM9. At a lower dose of 20 μM for 24 hours, this compound does not cause DNA damage in HuH7 cells, though some damage is observed in LM9 cells. Its ability to inhibit cell proliferation in both LM9 and HuH7 cells is concentration-dependent, evident from treatments lasting 1-2 weeks with 1.25-8 μM and from blocking the cell cycle at the G2/M phase at 0-20 μM for 48 hours. Additionally, this inhibitor reduces the migration and invasion of these cells and downregulates the expression of matrix metalloproteinases-9 (MMP-9) in a concentration-dependent manner, with significant effects noticeable at dosages ranging from 0-20 μM over 24 to 48 hours. Notably, concentrations of 3.5-20 μM over 48 hours can damage mitochondrial function and induce apoptosis in these cells. Investigations into its anti-proliferative activity across various cell lines (including LM9, HuH7, SK-hep-1, HepG2, HT-29, HCT-116, RKO, SW480, MCF-7, MDA-B-231, HGC-27, SGC-7901, BGC-823, A549, U251, HL-60, LO2) confirmed its efficacy, especially in HuH7 and LM9. Further analyses through Western Blot and cell cycle assessments corroborate its effectiveness in inhibiting MMP-9 expression and arresting the cell cycle at the G2/M phase, respectively, thus highlighting its potential as a targeted therapy in human hepatocellular carcinoma treatment.

Molecular Weight	336.34
Formula	C19H16N2O4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Topoisomerase I/II inhibitor 2 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.