SHP2-IN-38 is an innovative green fluorescent inhibitor targeting SHP2, exhibiting IC50 values as follows: 2.89 μM (SHP2), 8.73 μM (SHP1), 11.08 μM (PTP1B), and 33.07 μM (TCPTP). It impedes the SHP2-mediated ERK signaling pathway and inhibits MV4-11 cell proliferation in vitro with an IC50 of 7.90 μM. The excitation wavelength of SHP2-IN-38 is 360 nm, with a maximum emission wavelength of 550 nm in DMSO and 540 nm in DMF. Additionally, it demonstrates green fluorescent imaging in HeLa cells and zebrafish.

SHP2-IN-38 exhibits significant inhibitory effects on the proliferation of MV4-11 cells with an IC₅₀ value of 7.90 μM when used at concentrations between 0-20 μM over 3 days. At 20 μM for 24 hours, it reduces the phosphorylation levels of p-ERK in MV4-11 cells. The compound has two distinct absorption peaks at 320 nm and 360 nm, with 360 nm being the optimal excitation wavelength. Its maximum emission wavelength is 550 nm in DMSO and 540 nm in DMF. Furthermore, SHP2-IN-38 at 20 μM for 16 hours provides excellent fluorescence imaging in HeLa cells.

SHP2-IN-38 (1 h) demonstrates effective fluorescence imaging in zebrafish.