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RTI-13951-33 hydrochloride

Name: RTI-13951-33 hydrochloride
Brand: TargetMol
SKU: T12778
Rating: 4.5 (1 reviews)

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Catalog No. T12778

Alias RTI-13951-33 hydrochloride (2244884-08-8 free base)

RTI-13951-33 hydrochloride is a potent, selective, and brain-penetrant GPR88 agonist, exhibiting an EC50 of 25 nM in vitro cAMP functional assay. It significantly reduces alcohol reinforcement and intake behaviors in rats [1].

RTI-13951-33 hydrochloride

😃Good

Catalog No. T12778Alias RTI-13951-33 hydrochloride (2244884-08-8 free base)

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	Inquiry	3-6 months	3-6 months
50 mg	Inquiry	3-6 months	3-6 months
100 mg	Inquiry	3-6 months	3-6 months

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	RTI-13951-33 hydrochloride is a potent, selective, and brain-penetrant GPR88 agonist, exhibiting an EC50 of 25 nM in vitro cAMP functional assay. It significantly reduces alcohol reinforcement and intake behaviors in rats [1].
Targets&IC50	GPR88:（EC50）25 nM
In vitro	RTI-13951-33 is a potent and selective GPR88 agonist that effectively crosses the blood-brain barrier, demonstrating a significant effect with an EC50 value of 25 nM in GPR88 cAMP functional assays. It also increases [35S]-GTPγS binding in the mouse striatum with an EC50 of 535 nM, a response absent in GPR88 knockout (KO) mice. Despite its primary activity, RTI-13951-33 exhibits weak affinities for the kappa opioid receptor (KOR; Ki, 2.29 μM), the vesicular monoamine transporter (VMAT; Ki, 4.23 μM), and a moderate affinity for the serotonin transporter (SERT; Ki, 0.75 μM). However, it demonstrates poor inhibition of SERT, with an IC50 of 25.1±2.7 μM.
In vivo	RTI-13951-33 (10 and 20 mg/kg, i.p.) reduces alcohol lever responses in a rat model of alcohol self-administration dose-dependently, demonstrating sufficient brain penetration with a half-life of 48 minutes in plasma and 87 minutes in brain [1].
Synonyms	RTI-13951-33 hydrochloride (2244884-08-8 free base)

Chemical Properties

Molecular Weight	532.5
Formula	C₂₈H₃₅Cl₂N₃O₃
Smiles	COCC1=CC=C(C2=CC=C(N(C[C@@H](N)[C@@H](C)OC)C([C@H]3[C@H](C4=CC=CC=N4)C3)=O)C=C2)C=C1.Cl.Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 125 mg/mL (234.74 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (6.2 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8779 mL	9.3897 mL	18.7793 mL	93.8967 mL
5 mM	0.3756 mL	1.8779 mL	3.7559 mL	18.7793 mL
10 mM	0.1878 mL	0.9390 mL	1.8779 mL	9.3897 mL
20 mM	0.0939 mL	0.4695 mL	0.9390 mL	4.6948 mL
50 mM	0.0376 mL	0.1878 mL	0.3756 mL	1.8779 mL
100 mM	0.0188 mL	0.0939 mL	0.1878 mL	0.9390 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc