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Qstatin

Name: Qstatin
Brand: TargetMol
SKU: T5816
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5816Cas No. 902688-24-8

QStatin, a Selective Inhibitor of Quorum Sensing in Vibrio Species( SmcR with an EC50 of 208.9 nM).

Qstatin

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Purity: 99.99%

Catalog No. T5816Cas No. 902688-24-8

QStatin, a Selective Inhibitor of Quorum Sensing in Vibrio Species( SmcR with an EC50 of 208.9 nM).

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$45	In Stock	In Stock
25 mg	$83	In Stock	In Stock
50 mg	$117	In Stock	In Stock
100 mg	$167	In Stock	In Stock
200 mg	$243	-	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.99%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	QStatin, a Selective Inhibitor of Quorum Sensing in Vibrio Species( SmcR with an EC50 of 208.9 nM).
Targets&IC50	SmcR:208.9 nM(EC50)
In vitro	Because 377B6 [1-(5-bromothiophene-2-sulfonyl)-1H-pyrazole](QStatin) showed the strongest inhibition of SmcR activity without significant growth attenuation of V. vulnificus. SmcR activity was assessed by measuring the RLU level for V. vulnificus WT (pBB1) in the presence of different concentrations of QStatin, which revealed that the half-maximal effective concentration (EC50) of QStatin was 208.9 nM.
Cell Research	The plasmid pBB1 carrying the LuxRVh homologue-activated lux operon was conjugally transferred into the V. vulnificus wild-type (WT) strain, the ΔsmcR mutant, or the ΔluxO mutant. These V. vulnificus reporter strains were grown overnight, diluted 1:1,000 in fresh LBS, and treated with hit molecules. RLU values were calculated every hour. To determine the EC50, QStatin (10^?12 to 10^?4 M) or 2% DMSO as a control was added to the V. vulnificus WT reporter strain and RLU was measured after 5 h. The percentage of SmcR activity of the sample at a given concentration of QStatin was determined using the following equation: percent SmcR activity = sample RLU/control RLU × 100. The EC50 of QStatin (the concentration reducing the SmcR activity to 50%) was calculated from a plot of the percentages of SmcR activity versus QStatin concentrations using GraphPad Prism 6.0.

Chemical Properties

Molecular Weight	293.16
Formula	C₇H₅BrN₂O₂S₂
Cas No.	902688-24-8
Smiles	Brc1ccc(s1)S(=O)(=O)n1cccn1
Relative Density.	1.93 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 83.33 mg/mL (284.25 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (11.26 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4111 mL	17.0555 mL	34.1111 mL	170.5553 mL
5 mM	0.6822 mL	3.4111 mL	6.8222 mL	34.1111 mL
10 mM	0.3411 mL	1.7056 mL	3.4111 mL	17.0555 mL
20 mM	0.1706 mL	0.8528 mL	1.7056 mL	8.5278 mL
50 mM	0.0682 mL	0.3411 mL	0.6822 mL	3.4111 mL
100 mM	0.0341 mL	0.1706 mL	0.3411 mL	1.7056 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc