Pyranine (Solvent Green 7) is reported as a new class of fluorescent chemosensor for the Cu+ ion.

I. Cu2+ ion detection
1. Material preparation:
1) Pyranine solution: Pyranine is usually dissolved in an appropriate solvent, such as water, PBS or other buffer, to a concentration of 1-10 μM.
2) Cu+ ion solution: Prepare Cu+ ion solutions of different concentrations.
3) Fluorescence spectrometer: A device that can provide an excitation wavelength of 450 nm and an emission wavelength of 510 nm.
2. Steps:
1) Solution preparation: Prepare Pyranine solution and prepare solutions of different concentrations containing Cu+ ions.
2) Reaction: Add Pyranine solution to the solution containing Cu+ ions and mix thoroughly.
3) Fluorescence measurement: Use a fluorescence spectrometer to measure the fluorescence intensity at an excitation wavelength of 450 nm and an emission wavelength of 510 nm.
4) Data analysis: By measuring the fluorescence changes of Cu+ ions at different concentrations, the response of Pyranine in this environment is analyzed for quantitative analysis of Cu+ ion concentration.
2. pH sensitivity analysis
1. Material preparation:
1) Pyranine solution: As mentioned above, prepare a Pyranine solution of appropriate concentration.
2) pH buffer: Prepare buffers of different pH.
2. Steps:
1) Solution preparation: Prepare Pyranine solution and prepare buffers of different pH.
2) Measure pH changes: Add Pyranine solution to buffers of different pH and record its fluorescence intensity.
3) Fluorescence analysis: Analyze the response of Pyranine to pH based on the changes in fluorescence intensity.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

H2O: 100 mg/mL (190.7 mM), Sonication is recommended.

DMSO: 6.88 mg/mL (13.12 mM), Sonication is recommended.