Pyranine (Solvent Green 7) is reported as a new class of fluorescent chemosensor for the Cu+ ion.

I. Cu2+ ion detection
1. Material preparation:
1) Pyranine solution: Pyranine is usually dissolved in an appropriate solvent, such as water, PBS or other buffer, to a concentration of 1-10 μM.
2) Cu+ ion solution: Prepare Cu+ ion solutions of different concentrations.
3) Fluorescence spectrometer: A device that can provide an excitation wavelength of 450 nm and an emission wavelength of 510 nm.
2. Steps:
1) Solution preparation: Prepare Pyranine solution and prepare solutions of different concentrations containing Cu+ ions.
2) Reaction: Add Pyranine solution to the solution containing Cu+ ions and mix thoroughly.
3) Fluorescence measurement: Use a fluorescence spectrometer to measure the fluorescence intensity at an excitation wavelength of 450 nm and an emission wavelength of 510 nm.
4) Data analysis: By measuring the fluorescence changes of Cu+ ions at different concentrations, the response of Pyranine in this environment is analyzed for quantitative analysis of Cu+ ion concentration.
2. pH sensitivity analysis
1. Material preparation:
1) Pyranine solution: As mentioned above, prepare a Pyranine solution of appropriate concentration.
2) pH buffer: Prepare buffers of different pH.
2. Steps:
1) Solution preparation: Prepare Pyranine solution and prepare buffers of different pH.
2) Measure pH changes: Add Pyranine solution to buffers of different pH and record its fluorescence intensity.
3) Fluorescence analysis: Analyze the response of Pyranine to pH based on the changes in fluorescence intensity.