Pseudorabies virus-IN-1 is a potent inhibitor of pseudorabies virus (PRV) with an EC50 of 0.29 nM, acting by targeting PRV deoxyribonucleic acid polymerase (DNA pol) to suppress PRV replication. It effectively inhibits PRV replication even at low concentrations (MIC80: 1.6-8 nM) and is useful for research on PRV infections.

Pseudorabies virus-IN-1 (Compound C2) effectively inhibits 50% of PRV replication at a very low concentration (EC50: 0.29 nM) in PRV (MOI=1) infected PK15 cells over 48 hours, demonstrating significant activity. In PK15 cells, the compound shows relatively low cytotoxicity (CC50: 28 μM) over 72 hours at concentrations ranging from 0.05 to 100 μM.

Pseudorabies virus-IN-1 (Compound C2), administered intraperitoneally at 30 mg/kg three times daily for seven consecutive days, reduces the viral load in mouse brain tissue by over 99% compared to the control group (treatment group: 2.8×10³ - 2.3×10⁵ copies/g; control group: 9.5×10⁵ - 1.6×10¹⁰ copies/g). This regimen resulted in 100% survival in the treatment group, contrasting with 80% mortality in the control group, along with a significant reduction in viral copies, approximately threefold.