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Ponceau 4R

Name: Ponceau 4R
Brand: TargetMol
SKU: T16562
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16562Cas No. 2611-82-7

Alias New Coccine, Acid Red 18

Ponceau 4R (Acid Red 18) is a synthetic strawberry red azo dye that is a food colorant dye used in a variety of food products. It is stable to light, heat and acid, but fades in the presence of ascorbic acid. It is usually synthesized from aromatic hydrocarbons.

Ponceau 4R

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Purity: 97.70%

Catalog No. T16562Alias New Coccine, Acid Red 18Cas No. 2611-82-7

Pack Size	Price	USA Warehouse	Global Warehouse
1 g	$31	-	In Stock
5 g	$77	-	In Stock
1 mL x 10 mM (in DMSO)	$39	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.70%

Appearance:Solid

Color:Red

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Ponceau 4R (Acid Red 18) is a synthetic strawberry red azo dye that is a food colorant dye used in a variety of food products. It is stable to light, heat and acid, but fades in the presence of ascorbic acid. It is usually synthesized from aromatic hydrocarbons.
In vitro	Tissue Staining: 1. prepare a staining solution for Ponceau 4R (usually in water or an appropriate buffer at a concentration of 0.1%-1%). 2. for staining of tissue sections or certain biological samples, after which the stain is rinsed with an appropriate solution to remove background staining. 3. protein electrophoresis or immunoblotting experiments: under certain circumstances, Ponceau 4R may be used as a dye to detect protein bands, but is less commonly used for routine immunoblotting (WB) because its azo dye properties may interfere with certain experimental analyses." The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	New Coccine, Acid Red 18

Chemical Properties

Molecular Weight	604.46
Formula	C₂₀H₁₁N₂Na₃O₁₀S₃
Cas No.	2611-82-7
Smiles	O=S([O-])(C1=CC2=CC=C(C(/N=N/C3=CC=C(S(=O)([O-])=O)C4=C3C=CC=C4)=C2C(S(=O)([O-])=O)=C1)O)=O.[Na+].[Na+].[Na+]
Relative Density.	Ca. 1.772. Temperature:20 °C.

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 22.5 mg/mL (37.22 mM), Sonication is recommended.

DMSO: 9 mg/mL (14.89 mM), Sonication and heating to 60℃ are recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 0.5 mg/mL (0.83 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	1.6544 mL	8.2718 mL	16.5437 mL	82.7185 mL
5 mM	0.3309 mL	1.6544 mL	3.3087 mL	16.5437 mL
10 mM	0.1654 mL	0.8272 mL	1.6544 mL	8.2718 mL

H2O

	1mg	5mg	10mg	50mg
20 mM	0.0827 mL	0.4136 mL	0.8272 mL	4.1359 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc