Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PIPES (1,4-Piperazinediethanesulfonic acid) serves as a crucial component of the buffering agent in biochemical applications, known as PIPES buffer.
Description | PIPES (1,4-Piperazinediethanesulfonic acid) serves as a crucial component of the buffering agent in biochemical applications, known as PIPES buffer. |
In vitro | To prepare the PIPES buffer, dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water. Adjust the pH of the solution to 6.8 using sodium hydroxide pellets. This buffer is efficacious in mitigating lipid loss and artifacts induced by glutaraldehyde fixation [1]. |
Synonyms | 1,4-Piperazinediethanesulfonic acid, 1,4-piperazine bis(ethanesulfonic acid), 1,4-Piperazinebis(ethanesulfonic acid), PIPES |
Molecular Weight | 302.37 |
Formula | C8H18N2O6S2 |
CAS No. | 5625-37-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: < 1mg/ml (insoluble)
H2O: 5 mg/mL (16.5 mM), when pH is adjusted to 7 with NaOH. Sonication is recommended.
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
PIPES 5625-37-6 Others 1,4-Piperazinediethanesulfonic acid 1,4-piperazine bis(ethanesulfonic acid) 1,4-Piperazinebis(ethanesulfonic acid) inhibitor inhibit