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PIPES Disodium

Name: PIPES Disodium
Brand: TargetMol
SKU: T61146
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T61146 Copy Product Info

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PIPES (1,4-Piperazinediethanesulfonic acid) disodium is an amphoteric ion biological buffer agent commonly used to prepare buffers with a pH of 6.1 to 7.5, often employed in cell culture media, protein analysis, and electrophoresis experiments.

PIPES Disodium

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Catalog No. T61146

Cas No. 76836-02-7

Pack Size	Price	USA Stock	Global Stock	Quantity
500 g	$37	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description	PIPES (1,4-Piperazinediethanesulfonic acid) disodium is an amphoteric ion biological buffer agent commonly used to prepare buffers with a pH of 6.1 to 7.5, often employed in cell culture media, protein analysis, and electrophoresis experiments.
In vitro	To prepare the pH PIPES buffer, dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water and adjust the pH to 6.8 using sodium hydroxide pellets. This PIPES buffer formulation effectively mitigates lipid loss and artifacts induced by glutaraldehyde fixation [1].

Chemical Properties

Molecular Weight	324.35
Formula	C₈H₁₇N₂NaO₆S₂
Cas No.	76836-02-7
Smiles	O=S([O-])(CCN1CCN(CCS(=O)(O)=O)CC1)=O.[Na+]

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 50 mg/mL (154.15 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	3.0831 mL	15.4154 mL	30.8309 mL	154.1545 mL
5 mM	0.6166 mL	3.0831 mL	6.1662 mL	30.8309 mL
10 mM	0.3083 mL	1.5415 mL	3.0831 mL	15.4154 mL
20 mM	0.1542 mL	0.7708 mL	1.5415 mL	7.7077 mL
50 mM	0.0617 mL	0.3083 mL	0.6166 mL	3.0831 mL
100 mM	0.0308 mL	0.1542 mL	0.3083 mL	1.5415 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc