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PIPES Disodium

Name: PIPES Disodium
Brand: TargetMol
SKU: T61146
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T61146Cas No. 76836-02-7

PIPES (1,4-Piperazinediethanesulfonic acid) disodium is an amphoteric ion biological buffer agent commonly used to prepare buffers with a pH of 6.1 to 7.5, often employed in cell culture media, protein analysis, and electrophoresis experiments.

PIPES Disodium

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Catalog No. T61146Cas No. 76836-02-7

Pack Size	Price	Availability	Quantity
500 g	$37	In Stock

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Product Introduction

Bioactivity

Description	PIPES (1,4-Piperazinediethanesulfonic acid) disodium is an amphoteric ion biological buffer agent commonly used to prepare buffers with a pH of 6.1 to 7.5, often employed in cell culture media, protein analysis, and electrophoresis experiments.
In vitro	To prepare the pH PIPES buffer, dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water and adjust the pH to 6.8 using sodium hydroxide pellets. This PIPES buffer formulation effectively mitigates lipid loss and artifacts induced by glutaraldehyde fixation [1].

Chemical Properties

Molecular Weight	324.35
Formula	C₈H₁₇N₂NaO₆S₂
Cas No.	76836-02-7
Smiles	O=S([O-])(CCN1CCN(CCS(=O)(O)=O)CC1)=O.[Na+]

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 50 mg/mL (154.15 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	3.0831 mL	15.4154 mL	30.8309 mL	154.1545 mL
5 mM	0.6166 mL	3.0831 mL	6.1662 mL	30.8309 mL
10 mM	0.3083 mL	1.5415 mL	3.0831 mL	15.4154 mL
20 mM	0.1542 mL	0.7708 mL	1.5415 mL	7.7077 mL
50 mM	0.0617 mL	0.3083 mL	0.6166 mL	3.0831 mL
100 mM	0.0308 mL	0.1542 mL	0.3083 mL	1.5415 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc