Shopping Cart

Your shopping cart is currently empty

NCT02

Name: NCT02
Brand: TargetMol
SKU: T60030
Price: 58 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T60030Cas No. 790245-61-3

Alias NCT 02

NCT02 is a molecular glue degrader targeting CDK12 and CCNK (cyclin K), inducing CCNK ubiquitination and inhibiting CRC cell proliferation in vivo and in vitro, leading to apoptosis and cell cycle arrest. It is intended for the investigation of metastatic colorectal cancer (CRC).

NCT02

Copy Product Info

😃Good

Catalog No. T60030Alias NCT 02Cas No. 790245-61-3

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
2 mg	$58	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Appearance:Solid

Color:Yellow

Resource Download

COA

Product Introduction

Bioactivity

Description	NCT02 is a molecular glue degrader targeting CDK12 and CCNK (cyclin K), inducing CCNK ubiquitination and inhibiting CRC cell proliferation in vivo and in vitro, leading to apoptosis and cell cycle arrest. It is intended for the investigation of metastatic colorectal cancer (CRC).
In vitro	NCT02 (0-20 μM, 72 hours) exhibited low cytotoxicity toward normal fibroblasts but showed dose-dependent inhibition of cell viability in colorectal cancer cell lines (TSC03, TSC08, TSC47, etc.) [1]. NCT02 (10 μM, 24-48 hours) induced apoptosis in TSC03 and TSC14 cells, as evidenced by a significant increase in the proportion of cleaved PARP-positive cells [1]. NCT02 (10 μM, 6 hours) triggered degradation of the CCNK/CDK12 complex, leading to a marked reduction in CCNK protein levels and decreased phosphorylation of p-Ser2 POLR2 [1].
Synonyms	NCT 02

Chemical Properties

Molecular Weight	312.39
Formula	C₁₇H₁₆N₂O₂S
Cas No.	790245-61-3
Smiles	Cc1cnc(NC(=O)Cc2coc3cc4CCCc4cc23)s1

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (128.05 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2011 mL	16.0056 mL	32.0113 mL	160.0563 mL
5 mM	0.6402 mL	3.2011 mL	6.4023 mL	32.0113 mL
10 mM	0.3201 mL	1.6006 mL	3.2011 mL	16.0056 mL
20 mM	0.1601 mL	0.8003 mL	1.6006 mL	8.0028 mL
50 mM	0.0640 mL	0.3201 mL	0.6402 mL	3.2011 mL
100 mM	0.0320 mL	0.1601 mL	0.3201 mL	1.6006 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc