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N-Decanoyl p-Nitroaniline

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Catalog No. T37217Cas No. 72298-63-6

N-Decanoyl p-nitroaniline (DepNA) is a nitroaniline fatty acid amide used to measure fatty acid amide hydrolase (FAAH) activity. FAAH, which accepts various amide head groups beyond the ethanolamine of its endogenous substrate anandamide (AEA), can hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. When exposed to FAAH activity, DepNA releases the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 410 nm), enabling fast and convenient FAAH activity measurement using a 96-well plate spectrophotometer.

N-Decanoyl p-Nitroaniline

N-Decanoyl p-Nitroaniline

😃Good
Catalog No. T37217Cas No. 72298-63-6
N-Decanoyl p-nitroaniline (DepNA) is a nitroaniline fatty acid amide used to measure fatty acid amide hydrolase (FAAH) activity. FAAH, which accepts various amide head groups beyond the ethanolamine of its endogenous substrate anandamide (AEA), can hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. When exposed to FAAH activity, DepNA releases the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 410 nm), enabling fast and convenient FAAH activity measurement using a 96-well plate spectrophotometer.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
5 mg$6235 days35 days
10 mg$10835 days35 days
25 mg$24335 days35 days
50 mg$42735 days35 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
N-Decanoyl p-nitroaniline (DepNA) is a nitroaniline fatty acid amide used to measure fatty acid amide hydrolase (FAAH) activity. FAAH, which accepts various amide head groups beyond the ethanolamine of its endogenous substrate anandamide (AEA), can hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. When exposed to FAAH activity, DepNA releases the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 410 nm), enabling fast and convenient FAAH activity measurement using a 96-well plate spectrophotometer.
Chemical Properties
Molecular Weight292.379
FormulaC16H24N2O3
Cas No.72298-63-6
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 25 mg/mL (85.51 mM), Sonication is recommended.
DMF: 25 mg/mL (85.51 mM), Sonication is recommended.
DMSO:PBS (pH 7.2) (1:2): 0.6 mg/mL (2.05 mM), Sonication is recommended.
Ethanol: 20 mg/mL (68.4 mM), Sonication is recommended.
Solution Preparation Table
Ethanol/DMSO/DMF
1mg5mg10mg50mg
5 mM0.6840 mL3.4202 mL6.8404 mL34.2022 mL
10 mM0.3420 mL1.7101 mL3.4202 mL17.1011 mL
20 mM0.1710 mL0.8551 mL1.7101 mL8.5505 mL
50 mM0.0684 mL0.3420 mL0.6840 mL3.4202 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.
1 Enter information below:
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2 Enter the in vivo formulation:
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

N-Decanoyl p-NitroanilineNDecanoyl pNitroanilineN Decanoyl p Nitroaniline
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