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MY33-3

Catalog No. T39677 CAS 2204280-41-9

Synonyms: MY33-3

MY33-3 is a powerful and specific inhibitor of receptor protein tyrosine phosphatase (RPTP)β/ζ, demonstrating an IC50 value of approximately 0.1 μM. Moreover, MY33-3 exhibits inhibitory activity against PTP-1B with an IC50 value of about 0.7 μM. Additionally, MY33-3 has been shown to effectively decrease ethanol consumption and alleviate neuroinflammation and cognitive dysfunction induced by Sevoflurane.

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MY33-3, CAS 2204280-41-9

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Description	MY33-3 is a powerful and specific inhibitor of receptor protein tyrosine phosphatase (RPTP)β/ζ, demonstrating an IC50 value of approximately 0.1 μM. Moreover, MY33-3 exhibits inhibitory activity against PTP-1B with an IC50 value of about 0.7 μM. Additionally, MY33-3 has been shown to effectively decrease ethanol consumption and alleviate neuroinflammation and cognitive dysfunction induced by Sevoflurane.
Targets&IC50	PTP1B:0.7 μM (IC50), RPTPβ/ζ:0.1 μM (IC50)
In vitro	MY33-3, at a concentration of 1 μM with a pretreatment time of 5 minutes, inhibits the ethanol-induced activation of TrkA and ALK in SH-SY5Y cells without altering the overall protein levels of TrkA and ALK. Furthermore, MY33-3, within a concentration range of 0.1-10 μM over 24 hours, reduces LPS-induced nitrite production and the increase of iNOS in BV2 microglial cells. In a cell viability assay conducted on SH-SY5Y cells with a 1 μM concentration of MY33-3 pretreated for 5 minutes and co-treated for 15 minutes, the compound decreased the ethanol-induced activation of TrkA and ALK, indicating no significant effect on the total protein levels of TrkA or ALK.
In vivo	Administered orally (p.o.) at a dosage of 60 mg/kg on days 3 and 4, MY33-3 notably decreases ethanol consumption in male C57BL/6J mice (8-10 weeks old) undergoing a two-bottle drinking in the dark (DID) procedure with 20% ethanol. This comparison highlights a significant reduction in ethanol intake from day 2 to day 3 and a diminished preference for the ethanol solution by day 3, without impacting the overall fluid consumption. Furthermore, MY33-3 (administered intraperitoneally (i.p.)) counteracts the decline in discrimination index and the deterioration of motor learning abilities induced by Sevoflurane, illustrating its potential in reversing cognitive impairments associated with substance exposure.

Synonyms	MY33-3
Molecular Weight	397.4
Formula	C16H13F6NS2
CAS No.	2204280-41-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Fernández-Calle R, et, al. Pharmacological inhibition of Receptor Protein Tyrosine Phosphatase β/ζ (PTPRZ1) modulates behavioral responses to ethanol. Neuropharmacology. 2018 Jul 15;137:86-95. 2. Fernández-Calle R, et, al. Role of RPTPβ/ζ in neuroinflammation and microglia-neuron communication. Sci Rep. 2020 Nov 20;10(1):20259. 3. Mao S, et, al. Pleiotrophin Potentiates Sevoflurane Anesthesia-induced Learning Deficits in Mice. J Mol Neurosci. 2022 Jan;72(1):48-55.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

MY33-3 2204280-41-9 MY-33-3 MY33 3 MY333 inhibitor inhibit

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