MOPS sodium salt (MOPS-Na) is a novel stabilizer maintaining the pH of mammalian cell culture media. It used as a buffer for the native structure of BSA against thermal denaturation.MOPS sodium salt(3-(N-morpholino)propanesulfonic acid sodium salt) is commonly used as a buffer in biology.

I. Cell Culture Buffer
1. Buffer Preparation: Dissolve MOPS-Na in distilled water and adjust the pH as needed (usually 7.4) to form a buffer.
2. Add to culture medium: Add MOPS-Na to the cell culture medium to help maintain a stable pH during culture. The final concentration of the buffer is usually 20 mM to 50 mM, which is adjusted according to the experimental requirements.
3. Sterilization: The buffer can be processed by autoclaving or filter sterilization to ensure its sterility and suitability for cell culture.
4. pH Monitoring: During cell culture, it is recommended to check the pH value regularly, especially during long-term culture or culture in a closed system, to prevent CO₂ accumulation from causing pH changes.
II. Electrophoresis
1. Prepare MOPS-based electrophoresis buffer, which usually contains 50 mM MOPS-Na, 50 mM sodium acetate and 1 mM EDTA, and adjust the pH to 7.0-7.5.
2. This buffer can be used for agarose gel or polyacrylamide gel electrophoresis and is suitable for the separation of nucleic acids and proteins.
III. In vitro biochemical reactions
Prepare MOPS buffer at an appropriate concentration (usually 20-50 mM) and adjust the pH according to the specific reaction conditions to ensure that the pH remains stable throughout the experiment.