MAPK-IN-5 is a potent inhibitor of MAPK, with an IC50 value of 1.35 μM in HeLa cells. It induces ROS-mediated DNA damage and mitochondrial apoptosis (apoptosis) via the MAPK pathway. MAPK-IN-5 significantly inhibits colony formation in HeLa cells, reduces viable cell numbers, suppresses cell migration, and arrests the cell cycle at the G2/M phase. It is applicable in cervical cancer research.

MAPK-IN-5 (Compound 3h) at concentrations of 5-10 μM over 48 hours significantly inhibits colony formation and increases cell death in HeLa cells. It notably hinders the migration of HeLa cells within 0-48 hours. Furthermore, MAPK-IN-5 at 5-10 μM for 24 hours can induce G2/M phase arrest. After 48 hours, it promotes apoptosis in HeLa cells by activating the mitochondrial apoptotic pathway. Additionally, MAPK-IN-5 stimulates ROS production, causes DNA damage, and modulates the MAPK signaling pathway in HeLa cells.