Lumiflavine (Lumilactoflavin) inhibits riboflavin uptake. It is produced by the photolysis of vitamin B2.

1. Detection of riboflavin uptake
1. Labeling and detection: First, add Lumiflavine to cultured cells or animal models. Because it shares an absorption mechanism with riboflavin, it can be absorbed by intracellular transporters, enter cells and accumulate inside cells. Then observe and analyze its fluorescence signal through a fluorescence microscope or fluorescence detector, thereby indirectly reflecting the cell's riboflavin uptake.
2. Cell experiment: Lumiflavine can competitively bind to intracellular riboflavin to help study the cell's ability to absorb vitamin B2. By comparing the effects of Lumiflavine with other known riboflavin uptake agents, the activity of transporters and the bioavailability of vitamin B2 can be evaluated.
2. For biochemical research
Lumiflavine can be used to study enzymes or pathways related to vitamin B2 metabolism. It can be used as an indicator of vitamin B2 metabolism, transport and deficiency status for the study of metabolic pathology.
3. Drug development
Lumiflavine can be used as a drug screening tool for studying vitamin B2 deficiency.