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Laflunimus

Name: Laflunimus
Brand: TargetMol
SKU: T11813
Rating: 4.5 (1 reviews)

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Catalog No. T11813Cas No. 147076-36-6

Alias HR325, HR 325

Laflunimus (HR325) is a DHODH inhibitor that inhibits immunoglobulin IgM and IgG secretion in vitro and in vivo, with an IC50=2.5 μm, and exerts an immunosuppressive effect by interfering with pyrimidine metabolism, and is used in the study of neuralgia.

Laflunimus

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Purity: 99.86%

Catalog No. T11813Alias HR325, HR 325Cas No. 147076-36-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$199	6-8 weeks	6-8 weeks
2 mg	$299	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.86%

Appearance:Solid

Color:Yellow

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Product Introduction

Bioactivity

Description	Laflunimus (HR325) is a DHODH inhibitor that inhibits immunoglobulin IgM and IgG secretion in vitro and in vivo, with an IC50=2.5 μm, and exerts an immunosuppressive effect by interfering with pyrimidine metabolism, and is used in the study of neuralgia.
Targets&IC50	IgM secretion with 50 μM uridine: 70 μM, IgG secretion from LPS-stimulated mouse splenocytes: 2.0 μM, IgG secretion with 50 μM uridine:60 μM, IgM secretion from LPS-stimulated mouse splenocytes: 2.5 μM
In vitro	Laflunimus (HR325) inhibited LPS-induced IgM and IgG secretion from mouse splenocytes, with IC₅₀ values of 2.5 μM and 2 μM, respectively.[1] The addition of exogenous uridine (50 μM) reversed this effect, increasing the IC₅₀ values to 70 μM for IgM and 60 μM for IgG, suggesting a pyrimidine depletion mechanism.[1] Laflunimus also suppressed LPS-induced κ light-chain surface expression in 70Z/3 cells, which was similarly reversed by uridine.[1]
In vivo	Laflunimus inhibited the secondary antibody response to sheep red blood cells (SRBC) in mice, with an ID₅₀ of 38 mg/kg orally.[1] Co-administration of uridine reversed immunosuppression induced by Laflunimus (50 mg/kg), whereas uridine alone had no effect and did not reverse immunosuppression caused by cyclophosphamide (10 mg/kg).[1]
Synonyms	HR325, HR 325

Chemical Properties

Molecular Weight	310.27
Formula	C₁₅H₁₃F₃N₂O₂
Cas No.	147076-36-6
Smiles	N(C(/C(=C(\O)/C1CC1)/C#N)=O)C2=CC(C)=C(C(F)(F)F)C=C2
Relative Density.	1.441g/cm3

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (128.92 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2 mg/mL (6.45 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2230 mL	16.1150 mL	32.2300 mL	161.1500 mL
5 mM	0.6446 mL	3.2230 mL	6.4460 mL	32.2300 mL
10 mM	0.3223 mL	1.6115 mL	3.2230 mL	16.1150 mL
20 mM	0.1611 mL	0.8057 mL	1.6115 mL	8.0575 mL
50 mM	0.0645 mL	0.3223 mL	0.6446 mL	3.2230 mL
100 mM	0.0322 mL	0.1611 mL	0.3223 mL	1.6115 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc