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Laflunimus

Name: Laflunimus
Brand: TargetMol
SKU: T11813
Price: 199 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11813Cas No. 147076-36-6

Alias HR325, HR 325

Laflunimus (HR325) is a DHODH inhibitor that inhibits immunoglobulin IgM and IgG secretion in vitro and in vivo, with an IC50=2.5 μm, and exerts an immunosuppressive effect by interfering with pyrimidine metabolism, and is used in the study of neuralgia.

Laflunimus

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Purity: 99.86%

Catalog No. T11813Alias HR325, HR 325Cas No. 147076-36-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$199	-	In Stock
2 mg	$299	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.86%

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Product Introduction

Bioactivity

Description	Laflunimus (HR325) is a DHODH inhibitor that inhibits immunoglobulin IgM and IgG secretion in vitro and in vivo, with an IC50=2.5 μm, and exerts an immunosuppressive effect by interfering with pyrimidine metabolism, and is used in the study of neuralgia.
Targets&IC50	IgG secretion from LPS-stimulated mouse splenocytes: 2.0 μM, IgM secretion from LPS-stimulated mouse splenocytes: 2.5 μM, IgG secretion with 50 μM uridine:60 μM, IgM secretion with 50 μM uridine: 70 μM
In vitro	Laflunimus (HR325) inhibited LPS-induced IgM and IgG secretion from mouse splenocytes, with IC₅₀ values of 2.5 μM and 2 μM, respectively.[1] The addition of exogenous uridine (50 μM) reversed this effect, increasing the IC₅₀ values to 70 μM for IgM and 60 μM for IgG, suggesting a pyrimidine depletion mechanism.[1] Laflunimus also suppressed LPS-induced κ light-chain surface expression in 70Z/3 cells, which was similarly reversed by uridine.[1]
In vivo	Laflunimus inhibited the secondary antibody response to sheep red blood cells (SRBC) in mice, with an ID₅₀ of 38 mg/kg orally.[1] Co-administration of uridine reversed immunosuppression induced by Laflunimus (50 mg/kg), whereas uridine alone had no effect and did not reverse immunosuppression caused by cyclophosphamide (10 mg/kg).[1]
Synonyms	HR325, HR 325

Chemical Properties

Molecular Weight	310.27
Formula	C₁₅H₁₃F₃N₂O₂
Cas No.	147076-36-6
Smiles	N(C(/C(=C(\O)/C1CC1)/C#N)=O)C2=CC(C)=C(C(F)(F)F)C=C2
Relative Density.	1.441g/cm3
Color	Yellow
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (128.92 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2 mg/mL (6.45 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2230 mL	16.1150 mL	32.2300 mL	161.1500 mL
5 mM	0.6446 mL	3.2230 mL	6.4460 mL	32.2300 mL
10 mM	0.3223 mL	1.6115 mL	3.2230 mL	16.1150 mL
20 mM	0.1611 mL	0.8057 mL	1.6115 mL	8.0575 mL
50 mM	0.0645 mL	0.3223 mL	0.6446 mL	3.2230 mL
100 mM	0.0322 mL	0.1611 mL	0.3223 mL	1.6115 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc