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JAK-2/3-IN-3

Catalog No. T60895

JAK-2-/3-IN-3 (compound ST4j) can be used in lymph derived diseases and leukemia research. JAK-2-/3-IN-3 inhibits JAK2 autophosphorylation and induces apoptosis in a time- and dose-dependent manner. JAK-2-/3-IN-3 is a potent inhibitor of JAK2/3 with IC50 values of 13.00 and 14.86 nM for JAK2 and JAK3, respectively [1].

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

JAK-2/3-IN-3, CAS N/A

Pack Size	Availability	Price/USD
25 mg	10-14 weeks	$ 1,520.00
50 mg	10-14 weeks	$ 1,980.00
100 mg	10-14 weeks	$ 2,500.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

JAK-2/3-IN-3, within a concentration range of 0-100 µM over 72 hours, selectively targets human erythroleukemia cells, demonstrating minimal toxicity towards normal cells. It exerts its effect primarily through inhibiting the JAK2/STAT5 signaling pathway, crucial for the proliferation of erythroleukemia TF1 cell line. Additionally, exposure to 15.53 µM of JAK-2/3-IN-3 induces apoptosis in a time-dependent manner within TF1 cells, culminating in increased apoptotic rates of approximately 20%, 40%, and 70% after 24, 48, and 72 hours, respectively. Further dose-dependent investigations reveal escalating apoptosis within TF1 cells at concentrations corresponding to IC 25, IC 50, and IC 75 over a 24-hour period, with noted apoptotic increases of approximately 15%, 20%, and 30%. In cell cytotoxicity assays involving TF1, HEL, Vero, and HepG2 cells treated with up to 100 µM of JAK-2/3-IN-3 for 72 hours, the compound demonstrated inhibition of TF1 and HEL cell growth with IC 50 values of 15.53 and 17.90 µM, respectively, while proving less toxic to normal Vero and HepG2 cells, with IC 50 values exceeding 100 µM for Vero cells and 50 µM for HepG2 cells.

Molecular Weight	325.15
Formula	C13H10Cl2N4O2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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You can also refer to dose conversion for different animals. More

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

JAK-2/3-IN-3 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.